Evaluation of Candidate Reference Genes for Normalization of Quantitative RT-PCR in Cinnamomum camphora Under Various Abiotic Stresses

doi:10.13560/j.cnki.biotech.bull.1985.2016.10.025

Abstract

Abstract: This study is to obtain the optimal reference genes of Cinnamomum camphora under different abiotic stresses. The expression level and expression stability of two candidate reference genes CcACTc and CcEF1α in C. camphora under various abiotic stresses were analyzed using quantitative real-time polymerase chain reaction（qRT-PCR）technique and BestKeeper software,respectively. The results indicated that,CcEF1α was relatively stable under low temperature treatment,and performed higher stability under other abiotic stress,such as drought,high salinity and ABA treatment compared to CcEF1α. Accordingly,CcEF1α is a considerable reference gene for quantitative analysis of stress-related genes in C. camphora.

Key words: Cinnamomum camphora, qRT-PCR, reference gene, abiotic stress

ZHANG Jia-jia, ZHANG Li-wei, LI Yong-peng, JIAO Xiao-lin, ZHU Feng-ling, DU Li. Evaluation of Candidate Reference Genes for Normalization of Quantitative RT-PCR in Cinnamomum camphora Under Various Abiotic Stresses[J]. Biotechnology Bulletin, 2016, 32(10): 205-211.

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