Functions of Detoxification and Application Prospect of Carboxypeptidase A

doi:10.13560/j.cnki.biotech.bull.1985.2017-0163

Abstract

Abstract: Ochratoxin A（OTA）is one of mycotoxins produced by Aspergillus and Penicillium，by which many products，such as cereals，beans，wines and coffees，are contaminated，and these contaminated foods possesses serious threats to food safety. In recent years，with the change of global climate，the food contaminated by OTA has become increasingly serious. In order to guarantee safety of humans and animals，many researchers are committing to detoxifying and degrading OTA in food and food raw materials. Currently，it is reported that OTA can be degraded by many fungi and bacteria，but few enzymes produced by them are characterized and purified，and degraded substances of OTA are unknown，thus they can’t be used in industry. Carboxypeptidase A（CPA）is an ideal product to degrade OTA because the detoxification ability of CPA to OTA is widely proved，its feature，structure and mechanism are very clear，and degraded product has no toxicity to human body；therefore，it is a novel approach to degrade OTA through genetic engineering and protein engineering to obtain a lot of stably and efficiently expressed CPA. This paper summarizes the contamination status of OTA in food，and mechanisms of bio-detoxification，the detoxification ability of CPA，and the application prospect of CPA as detoxifying agent，aiming at providing theoretical base for the development and application of mycotoxin agents degrading enzymes.

Key words: ochratoxin A, bio-detoxification, carboxypeptidase A

XIONG Lu, WANG Xiao-yun, LIANG Zhi-hong. Functions of Detoxification and Application Prospect of Carboxypeptidase A[J]. Biotechnology Bulletin, 2017, 33(8): 20-25.

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