It is well known that Botrytis cinerea is one of the most geographically widespread plant pathogen on tomato. A fungus BC2016-2 was successfully isolated from the tomato surface and identified as B. cinerea,a pathogen of tomato gray mold. Then,three strains with obvious inhibitory effects were screened using B. cinerea BC2016-2 as indicator fungus,and they were Bacillus amyloliquefacien CM3,Bacillus megaterium Y-30 and Bacillus amyloliquefaciens Y-48. The greenhouse pot experiment showed that the control effects of CM3,Y-30 and Y-48 on the tomato gray mold caused by B. cinerea BC2016-2 were 65.58%,54.1% and 72.13%,respectively.