Isolation and Identification of Indigenous Heterotrophic Denitrifying Bacteria from Polluted River

doi:10.13560/j.cnki.biotech.bull.1985.2017-0252

Abstract

Abstract: Heterotrophic denitrifying bacteria from the polluted water of Jialu River were screened on the selective medium，with sodium succinate as sole carbon source and ammonium sulfate as sole nitrogen source. Then，the separation and purification were performed by enrichment，gradient dilution and streaking on the plates. Finally，the strain was identified according to 16S rDNA sequence alignment results，physiological and biochemical experiments and the ammonia nitrogen transformation. The results showed that，63 strains were isolated from the polluted water，and 3 of them were identified as heterotrophic denitrifying bacterium，including a strain of Pseudomonas nirtoreducens and two strains of Pseudomonas mendocina，by which the ammonia nitrogen removal rate were 91.8%，89.8%，and 81.4%，respectively.

Key words: heterotrophic nitrification, indigenous bacteria, isolation, identification

LIU Pan-long, YU Lu-ji, LI Ting-mei, FAN Zheng, CHEN Tao. Isolation and Identification of Indigenous Heterotrophic Denitrifying Bacteria from Polluted River[J]. Biotechnology Bulletin, 2017, 33(10): 178-183.

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