Genome-wide Identification and Expression Characteristics of Invertase Gene Family in Jatropha curcas

doi:10.13560/j.cnki.biotech.bull.1985.2017-0303

Abstract

Abstract: Plant invertase is one of critical enzymes in sucrose metabolism and stress resistance formation. The 16 invertase genes were identified based on complete genome data of Jatropha curcas,then the evolutionary relationship,gene structure,physical and chemical characteristics,conserved motif,and expression feature were analyzed. The results showed that invertase gene family in J. curcas was clustered to three subfamilies according to phylogenetic analysis. The α subgroup of neutral/alkaline invertases and acidic invertase gene owned 6-7 exons,and β subgroup owned 4-5 exons in gene structure. Signal peptides in N-terminal and conserved motifs of -NDPNG/A- and -MWECV/P- belonging to GH32 family were identified in acidic invertase protein sequences. qRT-PCR analysis revealed that JcC-INVD expressed differently in different tissues,with abundantly in cotyledon,but scarcely in root,and remarkably cold-induced expression in leaf.

Key words: Jatropha curcas, invertase, gene family, expression analysis

WANG Hai-bo,XIN Hu,LIU Chao,TANG Li-zhou. Genome-wide Identification and Expression Characteristics of Invertase Gene Family in Jatropha curcas[J]. Biotechnology Bulletin, 2017, 33(9): 172-178.

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