This work is to select the suitable type and dosage of herbicide for highland barley in alpine region,and to determine the effects of different herbicides on the growth,yield and quality of highland barley. Using the single-factor experiment and random-plot selection method,we studied the control effects of herbicide Aixiu,Yanmaiwei,and Dabiaoma on main weed wild oats in highland barley field as well as the effects on the growth,yield and quality of barley in field conditions in Gannan alpine region. The treatments with 3 herbicides significantly reduced the number of weed wild oats,while some barley seedlings were a slightly yellow,but they recovered to normal after half a month. The best control effect of Aixiu was found in the 3-leaf stage of weed wild oats,as the average control effect per plant was 82% and the control effect in fresh weight reached 87%. The control effect with soil treatment of Yanmaiwei was poor and the Dabiaoma was the worst. The plant height,ear length,grain number per ear,and 1 000 grain weight of highland barley after different treatments were significantly or extremely higher than contrast. The yield increase of highland barley by Aixiu and Yanmaiwei treatments was obvious. There were no significant variations in protein contents,water contents and starch contents of highland barley among different treatments. In summary,Aixiu treatment presented better control effect on weed wild oats,increased yield significantly,and caused no impacts on the quality of highland barley,thus it could be considered as a suitable herbicide in highland barley field of alpine area.