Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (11): 54-59.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0404

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Advances on the Proteomics of Bovine Oocyte and Preimplantation Embryo Development

PU Li-ping, CHEN Fu-mei, ZHAO Xiu-ling, WANG Huan-jing, HOU Zhen XU, Zhuang-zhuang, ZHANG Peng-fei, ZHANG Ming   

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Animal Reproduction Institute,Guangxi University,Nanning 530004
  • Received:2017-05-18 Online:2017-11-26 Published:2017-11-22

Abstract: Proteomics,as a high-throughput method for displaying protein expression profiles of specific biological processes in a panoramic view,is being applied in more and more research fields. Bovine oogenesis and development of preimplantation embryo are inseparable from a series of changes in protein,thus the molecular mechanism of bovine oocyte maturation and embryo development can be investigated by proteomics. By establishing the proteomic expression profiles of oocytes before and after maturation as well as preimplantation embryos in different stages,we obtained marker proteins related to oocyte maturation and further validated and analyzed these markers,benefiting to understand the molecular mechanism of bovine oocyte maturation,in vitro fertilization and preimplantation embryo development. It lays a foundation for improving the mature rate of bovine oocyte,the rate of embryonic development and the rate of bovine reproduction. In this paper,the progress of proteomics in the bovine oocytes and development of preimplantation embryos was reviewed from the methods of studying proteomics,such as two-dimensional polyacrylamide gel electrophoresis,chromatography and mass spectrometry. To provide reference for further study of bovine oocyte and preimplantation embrv.

Key words: proteome, oocyte, preimplantation embryo