Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (12): 138-143.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0437

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Isolation and Identification of a Melanin-producing Marine Fungi and Characterization of the Pigment

HU Sheng-yuan2, LIU Shu1, 2, WANG Shu-jun1, JIAO Yu-liang1, 2, LAI Jiang-li2, GU Zhang-hui2 FANG Yao-wei1, 2,   

  1. 1. Jiangsu Marine Resources Development Research Institute,Lianyungang 222005;
    2. College of Fisheries and Marine Life,Huaihai Institute of Technology,Lianyungang 222005
  • Received:2017-05-26 Online:2017-12-25 Published:2017-12-21

Abstract: Our aims are to isolate melanin-producing fungi from Haizhou Bay,to clarify the taxonomic status,and to study the properties of the melanin. The pigments-producing fungi were isolated from the collected samples of sea water,sea anemone,and sea mud from Haizhou Bay with the method of enrichment culture. The melanin-producing fungi were re-screened by shake flask fermentation. Then the melanin-producing fungi were identified and the properties of the crudly extracted melanin were measured. The strain CXPF01 was obtained and identified as Cladosporium cladosporioides by its morphological and ITS sequence homology. The pigment had maximum absorption value at 220 nm. There was non-significant effect of light on the pigment while high temperature and alkalinity or acidity had a remarkable impact on it. The pigment presented inhibitory effect on Staphylococcus aureus and Escherichia coli. Conclusively,the melanin-producing fungus CXPF01 was obtained,from which the melanin was stable in light and it had antibacterial activity.

Key words: marine fungi, pigment, Cladosporium, property study.