Cloning and Functional Analysis of Green Tissue-specific Expression Promoter of Common Wild Rice(Oryza rufipogon Griff.)

doi:10.13560/j.cnki.biotech.bull.1985.2017-0443

Abstract

Abstract: Green tissue-specific expression promoter，which makes the exogenous gene express efficiently in green tissues of receptor crop. The green tissue-specific promoter OrGSP was cloned from Oryza rufipogon，and the fusion vector of OrGSP and GUS gene was constructed and transferred into Arabidopsis thaliana in order to identify the function of the promoter. Bioinformatics analysis shows that the length of 825 bp of OrGSP，contains the basic transcription initiation elements TATA-box and CAAT-box，and light responsive elements TCCC-motif，Sp1，G-box，I-box，GA-motif and as-2-box，etc. The GUS histochemical staining of transgenic Arabidopsis thaliana shows that OrGSP regulates the expression of GUS gene only in green tissue，and the GUS activity in leaf and stem is significantly higher than in roots. The promoter of Oryza rufipogon，OrGSP，is a green tissue-specific promoter and the results can provide new regulatory elements for crop molecular breeding.

Key words: promoter, green tissue-specific expression, common wild rice

ZHAO Zhi-qiang, XUE Man-de, HUANG Ke, ZHANG Jing-wen, LONG Yan, PEI Xin-wu, YUAN Qian-hua. Cloning and Functional Analysis of Green Tissue-specific Expression Promoter of Common Wild Rice(Oryza rufipogon Griff.)[J]. Biotechnology Bulletin, 2017, 33(8): 51-57.

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