Cloning and Expression Analysis of Cinnamate 4-hydroxylase Gene from Glycyrrhiza uralensis Suspension Cultured Cells

doi:10.13560/j.cnki.biotech.bull.1985.2017-0466

Abstract

Abstract: According to the reported C4H（cinnamate 4-hydroxylase）gene sequence（GenBank accession ID：D87520.1）from Glycyrrhiza echinata,the coding sequence of C4H gene from Glycyrrhiza uralensis Fisch suspension cultured cells was obtained by RT-PCR cloning technology,and the bioinformatics analysis on it was carried out. In addition,the expression patterns of C4H gene induced by methyl jasmonate（MeJA）were analyzed by q-PCR. The length of the cloned C4H’s open reading frame（ORF）was 1 515 bp,encoding 504 amino acids. The q-PCR experiments showed that C4H gene was induced by MeJA and reached the highest expression level at 12 h after MeJA added into the cell suspension system.

Key words: cinnamate 4-hydroxylase, licorice cells, methyl jasmonate, expression analysis

ZOU Guang-ping, LI Ya-li, FENG Meng-wei, YANG Xiu, XU Zhi-wei. Cloning and Expression Analysis of Cinnamate 4-hydroxylase Gene from Glycyrrhiza uralensis Suspension Cultured Cells[J]. Biotechnology Bulletin, 2017, 33(11): 96-100.

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