Abstract: This study aims to identify a mold by morphological observation,rDNA ITS sequence analysis,and Biolog microbial identification system,to investigate the growth of the mold and its consumption of carbon source by calculating the average well color development（AWCD）,and to analyze carbon source utilization of the mold using data. Results revealed as such,combined rDNA ITS sequence with morphological observation and Biolog identification system,the strain was identified as Aspergillus niger. Compared the consumptions of 8 carbon sources,the types of amino acid,lipid and acid were the most widely used. According to the utilizations of carbon sources,15 first-used carbon sources were listed. The optimal carbon source of the strain was P-hydroxyphenylacetic acid,which could be used as a material for the research of acid-resistant Aspergillus metabolism.

Key words: morphological observation, rDNA ITS sequence analysis, carbon metabolic, Biolog microbial identification system