Responses of Asperigillus niger to Oxidative Stress Under H2O2 Exposure

doi:10.13560/j.cnki.biotech.bull.1985.2018-0026

Abstract

Abstract: To investigate the response of Aspergillus niger to oxidative stress by adding H₂O₂ to the medium will provide the basis for controlling the mycelial growth and ochratoxin A（OTA）biosynthesis of A. niger. The effects of H₂O₂ on the growth,OTA biosynthesis,intracellular reactive oxygen species（ROS）formation,lipid peroxidation level,and antioxidase activities in A. niger were investigated. H₂O₂ inhibited the mycelial growth of A. niger,and the inhibition was dose dependent. Also H₂O₂ promoted OTA biosynthesis in A. niger,especially the most remarkably at the day 4 of growth. H₂O₂ caused intracellular ROS and malondialdehyde（MDA）content increasing in A. niger. H₂O₂ enhanced the activities of intracellular catalase（CAT）and glutathione peroxidase（GPX）,while significantly at the day 5 of growth for CAT and at the day 6 for GPX in A. niger. However,H₂O₂ presented no significant effect on the activity of superoxide dismutase（SOD）in A. niger. A. niger remediates oxidative stress by enhancing the activity of antioxidant enzymes and producing OTA,which may maintain the growth and metabolism of A. niger under oxygen stress.

Key words: Aspergillus niger, ochratoxin A, reactive oxygen species, antioxidase, oxidative stress

CHEN Hao-yu, XU Rui-tao, CHENG Zhi-xiang, GAO Qiang, ZHANG Jian. Responses of Asperigillus niger to Oxidative Stress Under H₂O₂ Exposure[J]. Biotechnology Bulletin, 2018, 34(4): 201-207.

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Responses of Asperigillus niger to Oxidative Stress Under H₂O₂ Exposure

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