Research Progresses on the Development and Application of CRISPR Technology

doi:10.13560/j.cnki.biotech.bull.1985.2017-0608

Abstract

Abstract: CRISPR,an adaptive immune defense system based on RNA,whose space sequence has homologous with the sequences of phage or plasmid,can use RNA that has specificity in target point to guide the Cas proteins to target the genetic error genes in almost all organisms and cells. This gene editing technique,compared with zinc finger nuclease（ZFN）and transcription activator-like effector nuclease（TALEN）,is cheaper and more efficient. Therefore,since its first application in 2013,CRISPR has been accepted quickly and applied to the studies by the masses of Chinese and foreign researchers,becoming a new hot spot in today's life science research. Regarding the great significance of CRISPR in future scientific studies and practical applications,this article reviews the recent research progress on CRISPR and its applications in gene therapy,and discusses the limitations of this technology and the solutions. Finally,the foreground of CRISPR is prospected for providing useful reference for relevant researchers.

Key words: CRISPR, Cas protein, gene therapy

LIANG Li-qin, YAN Jing, ZHANG Xin, HAO Ze-ting, DUAN Jiang-yan. Research Progresses on the Development and Application of CRISPR Technology[J]. Biotechnology Bulletin, 2018, 34(5): 9-16.

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