Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (4): 49-58.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1163

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CRISPR-associated Transposases and Their Applications in Bacterial Genome Editing

ZHOU Xiao-jie1,2(), YANG Si-qi1,2, ZHANG Yi-wen3, XU Jia-qi4, YANG Sheng1()   

  1. 1. CAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032
    2. University of Chinese Academy of Sciences, Beijing 100049
    3. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308
    4. ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou 311215
  • Received:2022-09-21 Online:2023-04-26 Published:2023-05-16

Abstract:

CRISPR-Cas can target a specific sequence of DNA or RNA guided by a short RNA guide. The target sites can be changed with the RNA sequences. A variety of powerful genetic tools have been developed based on this reprogramming property. Recently, it has been found that some CRISPR elements are captured by Tn7 transposons during evolution, the derived CRISPR-associated transposases(CASTs)system has the ability of RNA-guided DNA integration. Some of them has been deployed as programmable genome integration tools, which have broad application prospects in large fragments and multiple gene integration. This review traces the discovery of CASTs, summarizes the characteristics of different types of CASTs loci, the mechanism models of gene integration, and their deployments and applications in a variety of Gram-negative bacteria.

Key words: CRISPR-associated transposases, mobile genetic element, genome editing, multiplexed gene integration