Genome-wide Identification of MYB Gene Family and Cloning & Expression Analysis of JcMYB308 Gene from Jatropha curcas

doi:10.13560/j.cnki.biotech.bull.1985.2017-0621

Abstract

Abstract: MYB transcription factor family involves in the processes of the plant growth and development and the response to environmental stresses. Based on the database of Jatropha curcas genome,MYB308 gene family was identified,then JcMYB308 of J. curcas was cloned,and the functional domains,phylogenetic relationship,gene structure,and chilling expression characteristics were analyzed. The results demonstrated that the whole genome was identified to have 213 members of gene family,and they were clustered as 6 subfamilies. The cloned JcMYB308 gene fragment length was 713 bp,and the gene structure was highly conserved,with two exons. In addition,the phylogenetic tree showed that its amino acid sequence shared 62.7% identity with Ricinus communis that belonged to the same family of Euphorbiaceae. qRT-PCR expression analysis revealed that JcMYB308 expressed in different organs,abundantly in root,but scarcely in leaves,and at the highest level in stem and root at 24 h after cold-induction.

Key words: Jatropha curcas, MYB, gene family, MYB308, gene cloning, expression analysis

XIN Hu, YAN Yue-hui, DING Xue-mei, LIU Chao, GAO Yong, DAI Dong-qin, TANG Li-zhou, WANG Hai-bo. Genome-wide Identification of MYB Gene Family and Cloning & Expression Analysis of JcMYB308 Gene from Jatropha curcas[J]. Biotechnology Bulletin, 2018, 34(1): 110-118.

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