Preliminary Study on Physiological Function of Gene Bph14 in Rice

doi:10.13560/j.cnki.biotech.bull.1985.2017-0754

Abstract

Abstract: Gene Bph14 is the first cloned rice insect-resistant gene，however，there were few reports about the effects of Bph14 gene on rice physiology. The study of the physiological effects of the gene on rice would contributes to a comprehensive understanding of the resistance mechanism of gene Bph14 to brown planthopper. The gene Bph14 was transferred into rice cultivar m5274，and genes related to ethylene biosynthesis and abiotic stress response were detected by Q-PCR in transgenic lines carrying gene Bph14. In addition，transgenic lines and the accepter line were treated with 5 μmol/L ABA and 250 mmol/L NaCl respectively. In these lines，OsACO2，OsACS2，OsACS6，O1gP5CS，and O5gP5CS expressed differently from those in m5274. Genes such as CatA，CatB，CatC，RAB16A，LEA3，LIP9，SalT，and AdhI related to abiotic stress of drought and salt-resistance also differently expressed from those in m5274. At the same time，the introduction of Bph14 gene improved the sensitivity of rice to ABA and the resistance to salt stress. These results indicate that gene Bph14 in rice probably participates in the regulation of ethylene biosynthesis and expression of functional genes in abiotic stress response.

Key words: Bph14 gene, ethylene biosynthesis, abiotic stress, rice

LI San-he, ZHA Wen-jun, CHEN Zhi-jun, ZHOU Lei, LIU Kai, YOU Ai-qing. Preliminary Study on Physiological Function of Gene Bph14 in Rice[J]. Biotechnology Bulletin, 2017, 33(12): 93-98.

References

［1］Normile D. Reinventing rice to feed the world［J］. Science, 2008, 321（7）：330-333.
［2］Pathak MD, Cheng CH, Fortuno ME. Resistance to Nephotettix impicticeps and Nilaparvata lugens in varieties of rice［J］. Nature, 1969, 223（5205）：502-504.
［3］Jung JK, Im DJ. Feeding inhibition of the brown planthopper, Nilaparvata lugens（Homoptera：Delphacidae）on a resistant rice variety［J］. J Asia-Pacific Entomol, 2005, 8（3）：301-308.
［4］Cohen MB, Alam SN, Medina EB, Bernal CC. Brown planthopper, Nilaparvata lugens, resistance in rice cultivar IR64：Mechanism and role in successful N lugens management in Centrl Luzon, Philippines［J］. Entomol Exp Appl, 1997, 85（3）：221-229.
［5］Cuong NL, Ben PT, Phuong LT, Chau LM, Cohen MB. Effect of host plant resistance and insecticide on brown planthopper Nilaparvata lugens（St?l）and predator population development in the Mekong Delta, Vietnam［J］. Crop Prot, 1997, 16（8）：707-715.
［6］Khush GS. Green revolution：The way forward［J］. Nat Rev Genet, 2001, 2（10）：815-822.
［7］Du B, Zhang W, Liu B, et al. Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice［J］. Proc Natl Acad Sci USA, 2009（106）：22163-22168.
［8］Zhao Y, Huang J, Wang ZZ, et al. Allelic diversity in an NLR gene BPH9 enables rice to combat planthopper variation［J］. Proc Natl Acad Sci USA, 2016, 113（45）：12850-12855.
［9］Liu Y, Wu H, Chen H, et al. A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice［J］. Nat Biotechnol, 2015, 33（3）：301-305.
［10］Wang B, Huang Z, Shu LH, et al. Mapping of two new brown planthopper resistance genes from wild rice［J］. Chinese Science Bulletin, 2001, 46（13）：1092-1095.
［11］Huang Z, He GC, Shu LH, et al. Identification and mapping of two brown planthopper resistance genes in rice［J］. Theor Appl Genet, 2001, 102：929-934.
［12］苏金, 朱汝财. 渗透胁迫调节的转基因表达对植物抗旱耐盐性的影响［J］. 植物学通报, 2001, 18（2）：129-136.
［13］ Hmida-Sayari A, Cargouri-Bouzid R, Bidani A, et al. Overexpres-sion of Δ1-pyrroline-5-carboxylate synthetase increases proline producyion and confers salt tolerance in transgenic potato plants［J］. Plant Science, 2005, 169（4）：746-752.
［14］De Ronder JA. Photosynthetic response of transgenic soybean, containing an Arabidopsis P5CR gene, during heat and drought stress［J］. Journal of Plant Physiology, 2004, 161（11）：1211-1224.
［15］Hbc M, Marur CJ, Bespalhok FJC, et al. Osmotic adjustment in transgenic citrus root-stock Carrzo citrange（Citrus sinensis Osb. ×Poncirus trifoliate L. Raf）overproducting proline［J］. Plant Science, 2005, 167（6）：1375-1381.
［16］Nayyar H, Gupta D. Differential sensitivity of C3 and C4 plants to water deficit stress：Association with oxidative stress and antioxidants［J］. Environmental and Experimental Botany, 2006, 58：106-113.
［17］Fukao T, Yeung E, Bailey-Serres J. The submergence tolerance regulator SUB 1 A mediates crosstalk between submergence and drought tolerance in rice［J］. Plant Cell, 2011, 23（1）：412-427.
［18］Ganguly M, Datta K, Roychoudhury A, et al. Overexpression of Rab16A gene in indica rice variety for generating enhanced salt tolerance［J］. Plant Signaling ＆ Behavior, 2012, 7（4）：502-509.
［19］Hong CY, Chao YY, Yang MY, et al. NaCl-induced expression of glutathione reductase in roots of rice（Oryza sativa L.）seedlings is mediated through hydrogen peroxide but not abscisic acid［J］. Plant and Soil, 2009, 320（1-2）：103-115
［20］Duan JL, Cai W. OsLEA3-2, an abiotic stress induced gene of rice plays a key role in salt and drought tolerance［J］. PLoS One, 2012, 7（9）：e45117.
［21］Checker VG, Chhibbar AK, Khurana P. Stress-in-ducible expression of barley HvIII gene in transgenic mulberry displays enhanced tolerance against drought, salinity and cold stress［J］. Transgenic Res, 2012, 21（5）：939-957.
［22］ Zeevaart JAD, Creelman RA. Metabolism and physiology of abscisic acid［J］. Annu Rev Plant Physiol Mol Biol, 1988, 39（4）：439-473.
［23］Pe?a-Cortès H, Willmitzer L, Sánchez-Serrano JJ. Abscisic acid mediates the wound induction but not developmental-specific expression of the proteinase inhibitor II gene family［J］. Plant Cell, 1991, 3（9）：963-972.
［24］Strommer J, Garabagi F. ADH and PDC：Key roles for enzymes of alcoholic fermentation［M］//Gerats T, Strommer J（eds）. Petunia：Evolutionary, Developmental and Physiological Genetics. New York：Springer-Verlag New York Inc., 2009, 71-84.
［25］刘威, 陈昊, 靳亚忠, 等. 高等植物醇脱氢酶及其基因家族研究进展［J］. 植物生理学报, 2014, 50（10）：1479-1493.
［26］Popko J, H?nsch R, Mendel RR, et al. The role of abscisic acid and auxin in the response of poplar to abiotic stress［J］. Plant Biology, 2010, 12（2）：242-258.
［27］Ganguly M, Datta K, Roychoudhury A, et al. Overexpression of Rab16A gene in indica rice variety for generating enhanced salt tolerance［J］. Plant Signal Behav, 2012, 7（4）：502-509.
［28］Lenka SK, Katiyar A, Chinnusamy V, et al. Comparative analysis of drought-responsive transcriptome in Indica rice genotypes with contrasting drought tolerance［J］. Plant Biotechnol J, 2011, 9（3）：315-327.
［29］Kim H, Lee K, Hwang H, et al. Overexpression of PYL5 in rice enhances drought tolerance, inhibits growth, and modulates gene expression［J］. J Exp Bot, 2014, 65（2）：453-464.

[1]	WANG Zi-ying, LONG Chen-jie, FAN Zhao-yu, ZHANG Lei. Screening of OsCRK5-interacted Proteins in Rice Using Yeast Two-hybrid System [J]. Biotechnology Bulletin, 2023, 39(9): 117-125.
[2]	WU Yuan-ming, LIN Jia-yi, LIU Yu-xi, LI Dan-ting, ZHANG Zong-qiong, ZHENG Xiao-ming, PANG Hong-bo. Identification of Rice Plant Height-associated QTL Using BSA-seq and RNA-seq [J]. Biotechnology Bulletin, 2023, 39(8): 173-184.
[3]	YAO Sha-sha, WANG Jing-jing, WANG Jun-jie, LIANG Wei-hong. Molecular Mechanisms of Rice Grain Size Regulation Related to Plant Hormone Signaling Pathways [J]. Biotechnology Bulletin, 2023, 39(8): 80-90.
[4]	LI Yu, LI Su-zhen, CHEN Ru-mei, LU Hai-qiang. Advances in the Regulation of Iron Homeostasis by bHLH Transcription Factors in Plant [J]. Biotechnology Bulletin, 2023, 39(7): 26-36.
[5]	ZHAO Xue-ting, GAO Li-yan, WANG Jun-gang, SHEN Qing-qing, ZHANG Shu-zhen, LI Fu-sheng. Cloning and Expression of AP2/ERF Transcription Factor Gene ShERF3 in Sugarcane and Subcellular Localization of Its Encoded Protein [J]. Biotechnology Bulletin, 2023, 39(6): 208-216.
[6]	LI Yuan-hong, GUO Yu-hao, CAO Yan, ZHU Zhen-zhou, WANG Fei-fei. Research Progress in the Microalgal Growth and Accumulation of Target Products Regulated by Exogenous Phytohormone [J]. Biotechnology Bulletin, 2023, 39(6): 61-72.
[7]	FENG Shan-shan, WANG Lu, ZHOU Yi, WANG You-ping, FANG Yu-jie. Research Progresses on WOX Family Genes in Regulating Plant Development and Abiotic Stress Response [J]. Biotechnology Bulletin, 2023, 39(5): 1-13.
[8]	LIANG Cheng-gang, WANG Yan, LI Tian, OHSUGI Ryu, AOKI Naohiro. Effect of SP1 on Panicle Architecture by Regulating Carbohydrate Remobilization [J]. Biotechnology Bulletin, 2023, 39(5): 152-159.
[9]	ZHAI Ying, LI Ming-yang, ZHANG Jun, ZHAO Xu, YU Hai-wei, LI Shan-shan, ZHAO Yan, ZHANG Mei-juan, SUN Tian-guo. Heterologous Expression of Soybean Transcription Factor GmNF-YA19 Improves Drought Resistance of Transgenic Tobacco [J]. Biotechnology Bulletin, 2023, 39(5): 224-232.
[10]	ZHOU Ding-ding, LI Hui-hu, TANG Xing-yong, YU Fa-xin, KONG Dan-yu, LIU Yi. Research Progress in the Biosynthesis and Regulation of Glycyrrhizic Acid and Liquiritin [J]. Biotechnology Bulletin, 2023, 39(5): 44-53.
[11]	YANG Chun-hong, DONG Lu, CHEN Lin, SONG Li. Characterization of Soybean VAS1 Gene Family and Its Involvement in Lateral Root Development [J]. Biotechnology Bulletin, 2023, 39(3): 133-142.
[12]	YANG Mao, LIN Yu-feng, DAI Yang-shuo, PAN Su-jun, PENG Wei-ye, YAN Ming-xiong, LI Wei, WANG Bing, DAI Liang-ying. OsDIS1 Negatively Regulates Rice Drought Tolerance Through Antioxidant Pathways [J]. Biotechnology Bulletin, 2023, 39(2): 88-95.
[13]	MIAO Shu-nan, GAO Yu, LI Xin-ru, CAI Gui-ping, ZHANG Fei, XUE Jin-ai, JI Chun-li, LI Run-zhi. Functional Analysis of Soybean GmPDAT1 Genes in the Oil Biosynthesis and Response to Abiotic Stresses [J]. Biotechnology Bulletin, 2023, 39(2): 96-106.
[14]	JIANG Min-xuan, LI Kang, LUO Liang, LIU Jian-xiang, LU Hai-ping. Advances on the Expressions of Foreign Proteins in Plants [J]. Biotechnology Bulletin, 2023, 39(11): 110-122.
[15]	XU Rui, ZHU Ying-fang. The Key Roles of Mediator Complex in Plant Responses to Abiotic Stress [J]. Biotechnology Bulletin, 2023, 39(11): 54-60.