Abstract: Resveratrol was extracted from grape seed by ultrasonic assisted two-phase aqueous method. The oxidative damage model was constructed by inducing LO2 cell with alcohol（100 mmol/L），and aging mice model was built via D-galactose. The superoxide dismutase（SOD），glutathione peroxidase（GSH-px）activities and malondialdehyde（MDA）content in the cells and mice liver homogenate were measured after intervention by resveratrol extracts. Resveratrol extraction yield was 2.408±0.088 mg/g when liquid ratio was 1∶10，leaching time was 40 min，ultrasonic time，power and temperature were 300 W，10 min and 50℃，and aqueous two-phase system was ethanol：ammonium sulphate = 1∶1. Resveratrol extracts affecting LO2 cell reduced cells MDA content and increased the activity of SOD and GSH-px induced by ethanol. Moreover，resveratrol extracts presented a certain inhibitory effects on liver MDA production，and increased the activities of SOD and GSH-px in aging model mice.

Key words: grape seed, resveratrol, LO2 cells, oxidative damage