Sequence Analysis on the CRISPR Sites of Lactobacillus bulgaricus

doi:10.13560/j.cnki.biotech.bull.1985.2017-0952

Abstract

Abstract: CRISPR（Clustered regularly interspaced short palindromic repeats）sequence in the genome of Lactobacillus is its important part to recognize and resist phage,and CRISPR and cas protein constitute the acquired immune system. Currently there are few studies on the CRISPR sequence of Lactobacillus. In this study,clone sequencing was performed in the CRISPR of 8 strains of L. bulgaricus from different sources,and then the evolutionary genetics analysis to the repetitive sequence was also conducted,and the secondary structure was predicted;moreover the homology analysis in the spacer sequence was also carried out. Results showed that the lengths of CRISPR in 8 different strains of L. bulgaricus varied,among which the longest one was 1820 bp with 30 spacer sequences,and the shortest one was 408 bp with 5 spacer sequences. Two different structures were found in the prediction on the secondary structure of CRISPR repetitive sequence：one was the typical RNA mainly based on “ring” shape,another was the one mainly based on “stylo” shape. The former one had typical crRNA after cleaving,while the function of the latter needs to be further studied. This paper laid the foundation for exploring the molecular mechanism of L. bulgaricus against phage and has great significance in screening anti-phage fermentative strains in the dairy industry via analyzing the CRISPR sequence of L. bulgaricus.

Key words: Lactobacillus bulgaricus, homology, CRISPR, spacer sequence, secondary structure

CHENG Na, CAI Zhen-hua, LÜ Jia-ping, JIA Zhen-hu, PANG Xiao-yang. Sequence Analysis on the CRISPR Sites of Lactobacillus bulgaricus[J]. Biotechnology Bulletin, 2018, 34(5): 87-93.

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