cDNA Cloning and Transcriptional Expression Analysis of OsMPK15 in Rice

doi:10.13560/j.cnki.biotech.bull.1985.2017-0978

Abstract

Abstract: MAPK gene involves in the rice’s responses to biotic and abiotic stress as well as its development and growth. Cloning rice OsMPK15 and preliminarily studying its expression would provide foundation for further its functional studies. In this study，RT-PCR was used to clone its cDNA coding region of OsMPK15 from rice Nipponbare roots，and quantitative real time PCR（qRT-PCR）to analyze the expression characteristics in various tissues and under different abiotic treatments. The results showed that the cDNA coding sequence of OsMPK15 isolated from rice Nipponbare root was identical with the results of bioinformatics prediction. Sequence analysis revealed that a highly conserved serine/threonine kinase domain in MAPK protein was located at the position from 13th to 304th in OsMPK15 amino acids sequence，and it belonged to group E of MAPK family. qRT-PCR results demonstrated that OsMPK15 gene was widely expressed in various tissues，especially accumulated in leaves and leaf sheaths at young panicle stage. The expression of OsMPK15 gene in seedling roots was up-regulated by drought and salt stress with different expression pattern. Meanwhile，the treatment with stress-related hormones ABA，JA and SA resulted in the responses and the highest up-regulation by ABA.

Key words: rice, OsMPK15 gene, transcriptional expression analysis, abiotic stress, plant hormones

SHI Jia YANG Dan-dan GE Hui-wen DU Jing-yao LIANG Wei-hong. cDNA Cloning and Transcriptional Expression Analysis of OsMPK15 in Rice[J]. Biotechnology Bulletin, 2018, 34(6): 66-72.

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