Expression Analysis of Ribosomal Protein Genes in Aspergillus flavus

doi:10.13560/j.cnki.biotech.bull.1985.2017-1049

Abstract

Abstract: Ribosome is a major organelle of protein synthesis in organism. Ribosomal protein is one of main components of ribosome. In order to explore the relationship between Aspergillus flavus ribosomal protein and its development,A. flavus were collected at mycelial stage,spore stage,and sclerotial stage. After total RNA extraction and the cDNA preparation,the expression levels of 74 genes of A. flavus ribosomal protein were detected by quantitative RT-PCR. The results showed that,none of the tested genes was expressed invariably at all three stages. Compared with mycelial stage,the expression levels of 54 ribosomal protein genes were up-regulated at the spore stage,while 2 genes were down-regulated. At sclerotial stage,the expression levels of 57 genes were up-regulated,while 12 genes were down-regulated. The GO functional analysis showed that the ribosomal protein genes were enriched into structural molecular activity,binding,cell,macromolecular complex,organelle,cell part,organelle part,cell process,and metabolic process. It can be concluded that ribosomal proteins are closely related to the growth and development of A. flavus.

Key words: Aspergillus flavus, ribosomal protein, aflatoxin, growth and development, quantitative RT-PCR

GENG Long-po, WANG Xin-wang, HUANG Lu-hua, DENG Ji-li, WANG Shi-hua, ZHANG Feng. Expression Analysis of Ribosomal Protein Genes in Aspergillus flavus[J]. Biotechnology Bulletin, 2018, 34(4): 194-200.

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