Identification and Biocontrol Characteristics of Streptomyces sp. 30702

doi:10.13560/j.cnki.biotech.bull.1985.2017-1083

Abstract

Abstract: This work is to identify a strain of Streptomyces sp. 30702 isolated from rhizosphere of Cephalotaxus hainanensis and to estimate its biocontrol traits. According to analysis of the 16S rRNA gene sequences and multilocus sequence analysis（MLSA）as well as comparison of physiological and morphological characteristics，the Streptomyces sp. 30702 was identified as Streptomyces solisilvae，a member of the Streptomyces violaceusniger clade. The strain was able to produce protease，cellulase，chitinase，β-glucanase，1-aminocyclopropane-1-carboxylic acid（ACC）deaminase，and siderophore，and also had traits of plant growth-promoting and phosphate solubilization. Additionally，the fermented extract of strain 30702 inhibited the spore germination and mycelial growth of Colletotrichum gloeosporioides（anthracnose of yam）. The results indicate that the Streptomyces strain 30702 possesses promising biocontrol characteristics, thus it has potential development and application values in the biocontrol of plant diseases.

Key words: Streptomyces, Streptomyces violaceusniger clade, biocontrol characteristics

CONG Zi-wen ,JIAO Jing-hua ,ZHOU Shuang-qing, HUANG Dong-yi ,WU Wen-qiang ,XU Yun ,XIA We,i ZHANG Rong-ping ,HUANG Xiao-long. Identification and Biocontrol Characteristics of Streptomyces sp. 30702[J]. Biotechnology Bulletin, 2018, 34(6): 190-198.

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