Effects of Novel Antifungalmycin N2 Crude Extract from Streptomyces sp. N2 on Germination of Rice Seed

doi:10.13560/j.cnki.biotech.bull.1985.2017-0337

Abstract

Abstract: To investigate the effects of a novel antifungalmycin N2（AN2）crude extract from Streptomyces sp. N2 on germination of rice seed,the multi-index of germination,amylase activity,soluble sugar contents,soluble protein contents and the contents of malondialdehyde（MDA）in rice seeds were measured using ultraviolet-visible spectrophotometry. The results showed that the influence of AN2 on each index was correlated with the treated AN2 concentration. It was found that AN2 at low concentration promoted rice seed germination,while it at high concentrations had an inhibitory effect. When treated with 0.23-1.15 μg/mL of AN2,the characteristics of seed germination was effectively improved,and the growth of seedlings was significantly promoted,especially under the treatment with the concentration of 0.58 μg/mL. In addition,the content of soluble sugar,soluble proteins and amylase activity of optimal concentration treated groups was increased significantly than control group by 28.27%,7.73% and 6.49%,respectively. In the 0.23-1.15 μg/mL of AN2 treatment group,the MDA content decreased significantly. Therefore,proper concentration treatments promote germination capacity,improve enhance material metabolism and enhance ability of osmotic regulation.

Key words: Streptomyces sp. N2, rice, seed germination, physiological characteristics

WU Zhi-ming,ZHONG Min,LU Cheng-jian,ZHU Man-ning,DU Qi-feng,LI Kun-tai. Effects of Novel Antifungalmycin N2 Crude Extract from Streptomyces sp. N2 on Germination of Rice Seed[J]. Biotechnology Bulletin, 2017, 33(9): 153-159.

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