The Advantages and Limitations of CRISPR/Cas9-based Gene Editing Technology

doi:10.13560/j.cnki.biotech.bull.1985.2017-1100

Abstract

Abstract: CRISPR/Cas9 is becoming a hot spot in genome editing,and it provides a more efficient way for gene targeting than zinc finger endonuclease（ZFN）and transcription activator-like effector nuclease（TALEN）. CRISPR/Cas9 system exists widely in bacteria and archaea,carrying out the adaptive immune responses specifically recognizing the invaded genetic elements from phage infection,plasmid conjugation and transformation as well as and degrading exogenous DNA. CRISPR/Cas9 is a precise editing technology by which the target sites are recognized by a segment of 20 bp small RNA. CRISPR/Cas9 has the advantages of simple design and operation,high efficiency,and broad generality,thus it is a novel and revolutionary precise gene editing technology. This review induced and summarized the aspects of the discovery of CRISPR/Cas,mechanisms,gene editing,and application limitations,aiming at providing references for understanding its mechanisms and applying precise gene editing technology.

Key words: CRISPR/Cas9, gene editing, off-target effects, sgRNA

WU Yan, HAO Ya-qiao, WEI Xuan, SHEN Qi, LIU Ye-fei, WANG Sheng-hou, ZHAO Hong-xin. The Advantages and Limitations of CRISPR/Cas9-based Gene Editing Technology[J]. Biotechnology Bulletin, 2018, 34(5): 1-8.

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