Research Progress on the Prediction of Protein Stability Based on Amino Acid Sequence and Simulated Structure

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.011

Abstract

Abstract: Stability is one of the important properties of protein. Protein’s stability is not only demanded in industry,but also the prerequisite for its deriving new functions in protein evolution. Improving the stability of protein,especially protein of unknown structure,is a very challenging task,i.e.,traditional protein modification methods such as directed evolution are time-consuming and laborious,while rational design is difficult to replicate by other researchers. At present,there are many reported predictors for protein stability. However,the majority of these tools require the pre-measurement of three-dimensional structure of protein,which restricts the stability prediction of protein in unknown structure. Recently,a number of tools to predict the effects of mutations on the stability of unknown proteins have been developed using amino acid sequences and simulated structures of proteins,here we introduce the research progress on them,aiming at providing guidance for protein engineering.

Key words: protein stability, predictors, amino acid sequence, protein structure simulation

YI Hua-Wei, TANG Xiao-Feng. Research Progress on the Prediction of Protein Stability Based on Amino Acid Sequence and Simulated Structure[J]. Biotechnology Bulletin, 2017, 33(4): 83-89.

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