The Application Potential of Ectomycorrhizal Fungus Pisolithus tinctorius Assisting Plant in Phytoremediation of Cu-contaminated Soils

doi:10.13560/j.cnki.biotech.bull.1985.2017.04.019

Abstract

Abstract: The purposes of this paper are to study copper tolerance of Pisolithus tinctorius（Pt）and the effects of Pt on host pine（Pinus thunbergii）seedlings growth,nutrients and heavy metal（Cu）absorption,and to probe its potential for mitigating Cu-contaminated soil. Pt was treated by Cu at concentrations of 0（control）,5,50,100,250,and 500 µmol/L in pure culture with the pot experiment. The growth,nutrients,and Cu concentrations of different parts of seedlings（inoculated with or without Pt）were analyzed. Results showed that Pt strain presented greater Cu tolerance,and its 50% tolerance index（TI）was about 200 µmol/L. Inoculation with Pt significantly improved the growth of host plant（F = 44.57,P = 0.003）and the contents of nutrients such as P and Ca in roots and stems compared with non-inoculated ones,thus mitigating the toxic damage of Cu to host pine. The extraction efficiency of heavy metals in the soil could be improved by increasing biomass of host plants,though Cu concentration in the shoots of inoculated seedlings was decreased. Besides,inoculation with Pt reduced the toxicity of Cu to host plants via reducing the soil exchangeable Cu content. Conclusively,Cu-tolerant fungus Pt may improve the growth and nutrient absorption of host seedlings,as well as reduce the toxicity of Cu to host,improve the extraction efficiency of P. thunbergii to Cu. It shows application potential in phytoremediation and vegetation restoration in Cu-contaminated soil.

Key words: Pisolithus tinctorius（Pt）, Pinus thunbergii, copper tolerance, extraction, vegetation restoration

WEN Zhu-gui, WANG Jie, TANG Yang-ze, SHI Liang, HONG Li-zhou, SHEN Zhen-guo, CHEN Ya-hua. The Application Potential of Ectomycorrhizal Fungus Pisolithus tinctorius Assisting Plant in Phytoremediation of Cu-contaminated Soils[J]. Biotechnology Bulletin, 2017, 33(4): 149-156.

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