Preparation of Selenized Derivatives from Tobacco Leaf Polysaccharides and Its Antioxidant Activity in Vitro

doi:10.13560/j.cnki.biotech.bull.1985.2019-0323

Abstract

Abstract: Using flue-cured tobacco leaves as raw material,the crude polysaccharide was extracted by ultrasound-assisted and refined polysaccharides were purified and selenization of them was done for derivatives. The derivatives were characterized by infrared spectroscopy and their degrees of selenization substitution were measured. On the basis of this,the antioxidative activity of tobacco leaf polysaccharide and selenized tobacco leaf polysaccharide were studied in vitro. The results showed that tobacco leaf polysaccharide TPP1 and TPP2 were isolated when the polysaccharide was eluted with 0.30 mol/L and 0.40 mol/L NaCl solution,respectively. The selenization substitution degree of the two polysaccharides was 0.20 and 0.24,respectively. The antioxidant activity of selenized tobacco leaf polysaccharides was significantly greater than that of tobacco leaf polysaccharides in vitro antioxidant experiments,and the greater the degree of substitution was,the more obviously the ·DPPH and ·OH radical scavenging rate increased.

Key words: tobacco leaf, polysaccharide extraction, selenium derivatization, anti-oxidation

ZHANG Hao, WEI Yue-wei, JI Xiao-ming, PAN Ting-ting, WANG Hui. Preparation of Selenized Derivatives from Tobacco Leaf Polysaccharides and Its Antioxidant Activity in Vitro[J]. Biotechnology Bulletin, 2019, 35(10): 89-94.

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