Abstract: Screening microbial strains that have promising response to semiconductor photoelectron brings broad application prospects in the study of interaction between minerals and microorganisms. Using the uranium tailing area soil，the microbial strains with photoelectron-response were screened in the media of containing 1/2，1/4，1/8，1/10，1/50，1/100，and 1/1 000 semiconductor material TiN under illumination condition. Then the strain was identified through its morphological，physiological and biochemical characteristics，and the molecular phylogeny. Ultraviolet-visible absorption spectra and three-dimensional fluorescence spectra were used to analyze the growth of the strain and the changes of protein，humic acid，and other metabolites. The strain Y-5 screened from soil was identified to be Alcaligenes faecalis，which had strong response to photoelectron of TiN. The optoelectronic effect was not obvious due to rich nutrient in the 1/10 substrate concentration；but in 1/50 and 1/100 low substrate concentration，the growth rate was accelerated by photoelectron due to poor nutrient，and the protein and humic acid increased correspondingly. A photoelectron-response microbial strain A. faecalis was screened from an uranium tailing area soil，and the photoelectron promoted its growth and metabolism. This method demonstrated certain feasibility in screening the photoelectron-response.

Key words: photoelectron, Alcaligenes faecalis, growth and metabolism, protein, humic acid