Biotechnology Bulletin ›› 2018, Vol. 34 ›› Issue (10): 182-186.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0295

• Orginal Article • Previous Articles     Next Articles

Expression Analysis of Pathogenic Genes During the Infection of Colletotrichum gloeosporioides to Mango

SU Chu-lian1, 2, KANG Hao1, 2, MEI Zhi-dong3, YANG Shi-you1, LIU Xiao-mei1, PU Jin-ji2, ZHANG He2   

  1. 1. Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228;
    2. Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture,Haikou 571101;
    3. Chengmai County Agriculture Bureau,Chengmai 571900
  • Received:2018-03-30 Online:2018-10-26 Published:2018-11-07

Abstract:

This work aims to study the differential expressions of pathogenicity-related genes during the process of infecting mango by Colletotrichum gloeosporioides. Quantitative real-time PCR was utilized to analyze the expression variations of 11 genes including PL and PGduring pathogen infecting mango leaves and fruits. The results showed that the PG and PL genes were highly expressed continuously and the expression of SIN3P gene was low,while the other genes expressed high at 6 h after infection and then decreased during the process of C. gloeosporioides infection to mango leaves. PL,PG,SDH,ECH and other genes highly expressed when the pathogen infected the fruit,while the rest of the genes either increased or decreased. These results indicate that the pathogenic genes express differentially while C.gloeosporioides infects different tissues,and play different roles in the different infection period at varied level.

Key words: Colletotrichum gloeosporioides, pathogenic genes, expression analysis