Isolation and Identification of Human Urine-derived Stem Cells

doi:10.13560/j.cnki.biotech.bull.1985.2018-0312

Abstract

Abstract: In order to establish an efficient and convenient system for the cultivation and identification of human urine-derived stem cells（hUSCs）,urine samples from young volunteers were collected under aseptic conditions to isolate and culture hUSCs. The clone assay,morphological observation,karyotype analysis,growth curve determination,surface marker detection,osteogenic and adipogenic differentiation were utilized to identify the hUSCs. The clones of hUSCs were in three types of fiber-like,pebble-like and silk-like. For the second passage hUSCs highly-expressed antigens CD44,CD73 and CD90,the positive rates were higher than 99.6%;while for the lowly-expressed antigens CD34 and CD45,the positive rates were less than 0.3%. For the sixth passage hUSCs highly-expressed antigens CD44 and CD73,and the positive rates higher than 99.5%;while for the lowly-expressed antigens CD34 and CD45,the positive rates were less than 0.5%. The sixth passage hUSCs still had strong proliferative ability and stable karyotype,and was induced to differentiate into osteocyte and adipocyte. By this research,the efficient and convenient hUSCs culture and identification systems are established.

Key words: human urine-derived stem cells, growth curve, surface marker, seed cell, regenerative medicine

WANG Fu-ping, ZHANG Feng, ZHAO Tong-biao. Isolation and Identification of Human Urine-derived Stem Cells[J]. Biotechnology Bulletin, 2018, 34(8): 190-198.

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