Establishment of Cell Suspension Culture System Based on the Leaf Callus in Noni

doi:10.13560/j.cnki.biotech.bull.1985.2017-0871

Abstract

Abstract: This work aims to get the secondary metabolites from the cell suspension culture system of Morinda citrifolia L.（noni）leaves. In the experiment,the cell suspension system of noni leaf was established based on the callus induced from the sterilized leaves and determination of the subculture cycle by changing the collocation of hormone in liquid medium and inoculation amount of callus,and measuring some relevant parameter with the process of the culture. Liquid medium was MS+2.0 mg/L NAA+0.2 mg/L KT.,and the initial inoculation amount of callus was 60 g/L. During the 14th -22nd d,cells were in the linear growth phase,then grew slowly,and reached a peak of 11.8×10⁵/mL at the 26th day,and then declined. The best cell viability appeared at 21st day when the OD₄₈₅=1.8. The primary cell was irregular rod-shaped and showed regular clusters of small cells and spheres when in the sixth generation. The best subculture cycle of noni cell suspension culture system was 22-26 d,and the cell survival rate in the liquid suspension culture of noni leaves was 77.9%. In conclusion,a stable cell suspension culture system of noni leaves is established in the experiment.

Key words: Morinda citrifolia L, callus, cell viability, growth curve

ZHANG Zheng-xue, LAN Zeng-quan, WU Tian. Establishment of Cell Suspension Culture System Based on the Leaf Callus in Noni[J]. Biotechnology Bulletin, 2018, 34(5): 142-147.

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