Antibiotic Resistance and Resistance Mechanism in Enterococcus faecium from Cold-water Fish in Aletai Prefecture of Xinjiang

doi:10.13560/j.cnki.biotech.bull.1985.2018-0339

Abstract

Abstract: The antibiotic resistance,resistance genes and virulence factors of Enterococcus faecium strains from cold-water fish that collected from Aletai prefecture in Xinjiang were analyzed to evaluate the safety of cold-water fish. Disc diffusion method was used to detect the antimicrobial susceptibility of E. faecium strains to 12 antibiotics. Resistance genes and virulence factors were amplified by PCR. All the 13 E. faecium strains from intestine of cold-water fish were not resistant to the 12 antibiotics,while 11 strains being intermediate to ciprofloxacin and 2 strains being intermediate to streptomycin. All the 13 strains harbored the ciprofloxacin-resistant gene gyrA,while 2 strains carried the ciprofloxacin-resistant gene parC. Among the 13 strains,one strain carried achromycin-resistant gene tetK,one contained gene tetL,and 4 harbored the gene tetM and tetL. The 13 strains presented negative to the virulence factors. The analysis of antibiotic resistance and virulence factors of E. faecium strains suggests that the consumption of cold-water fish does not have a potential risk to the health of consumers.

Key words: cold-water fish, Enterococcus faecium, ciprofloxacin

XIAO He, ZHANG Yan, WANG Yong-rui, NI Yong-qing. Antibiotic Resistance and Resistance Mechanism in Enterococcus faecium from Cold-water Fish in Aletai Prefecture of Xinjiang[J]. Biotechnology Bulletin, 2018, 34(10): 143-149.

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