Screening and Identification of a Lovastatin-producing Strain of Aspergillus versicolor and Preliminary Optimization of Fermentation Conditions

doi:10.13560/j.cnki.biotech.bull.1985.2018-0453

Abstract

Abstract: Total 86 Aspergillus strains were selected from different natural fermentation samples of food,soil,air and so on. Lovastatin yields of Aspergillus strains were measured by thin-layer chromatography（TLC）and high performance liquid chromatography（HPLC）methods. An Aspergillus strain（No. A-8）producing higher lovastatin was screened out,and the yield was of 58 g/L. Strain A-8 was identified as Aspergillus versicolor based on ITS sequence and morphology characteristics. Medium components and fermentation conditions of Aspergillus strain A-8 were optimized using single factor experiment. The results indicated that appropriate fermentation conditions were 28℃,initial pH 5.2,rotation speed 180 r/min,and inoculation size 10%. Optimal medium components were as follows：C source lactose,C content 100 g/L,N source peptone,N content 12 g/L,C/N 15：1.5. The lovastatin production of Aspergillus strain A-8 increased by 2.24 times and resulted in 130.04 μg/mL. The Aspergillus strain A-8 is one potential industrial strain.

Key words: Aspergillus versicolor, lovastatin, screening, identification, optimization

XU Chu-xuan, WANG Jia-qi, JIANG Dong-hua. Screening and Identification of a Lovastatin-producing Strain of Aspergillus versicolor and Preliminary Optimization of Fermentation Conditions[J]. Biotechnology Bulletin, 2018, 34(11): 210-215.

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