Transcriptome Sequencing and Analysis of Hepatopancreas from Carps Under Cold Stress

doi:10.13560/j.cnki.biotech.bull.1985.2018-0503

Abstract

Abstract: This work aims to detect the differentially-expressed genes（DEGs）of cold-tolerant and cold-sensitive carps and to understand the molecular response mechanisms for carps to adapt in cold environment. The transcriptome sequencing of hepatopancreas from cold-tolerant Songpu carp and a cold-sensitive Hebao carp were conducted and the enrichment analysis of DEG function and pathways were carried out. Totally,10 521 DEGs were detected via differentially-expressed analysis,and 5246 DEGs of them presented expression in cold-tolerant species. DEGS were enriched in 144 metabolic pathways including glycolysis/gluconeogenesis pathway. Five DEGs were selected to conduct qRT-PCR,and the correlation of the results between qRT-PCR and RNA-seq was 0.89.This study revealed that cold stress significantly changed the transcriptome of hepatopancreas in the experimental carp. The expressions of genes encoding the key enzymes of glycolysis/gluconeogenesis pathway were apparently higher than that in cold-sensitive ones,revealing that the functions of these genes facilitate the long-term cold-adaptation of the cold-tolerant carps.

Key words: Cyprinus carpio, cold tolerance, hepatopancreas, transcriptome sequencing, glycolysis/gluconeogenesis pathway

LIU Si-jia, TIAN Fei, ZHANG Cun-fang, QIAO Zhi-gang, ZHAO Kai. Transcriptome Sequencing and Analysis of Hepatopancreas from Carps Under Cold Stress[J]. Biotechnology Bulletin, 2018, 34(11): 168-178.

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