Effect of Warming After Toping on the Growth and Development of the Upper Leaves of Flue-cured Tobacco

doi:10.13560/j.cnki.biotech.bull.1985.2018-0776

Abstract

Abstract: By studying the effects of warming after topping on the amino acid content and enzyme activity related to growth and development of the upper leaves of flue-cured tobacco,we aim to provide a reference for promoting the growth and development of upper leaves and increasing the yield of tobacco leaves. Having flue-cured tobacco variety Yunyan 116 as an experimental materials,two treatments was set：the normal temperature（T1）and warming treatment（T2）. The hydroxyproline content and POD activity in cell wall as well as IAAO activity in the upper leaves of the two treatments were measured. The results showed that warming after toping effectively reduced the content of hydroxyproline,cell wall POD activity and IAAO activity in the upper leaves of tobacco leaves. The length,leaf width,leaf area,the yield and production value of the upper leaves for T2 treatment increased by 8.6%,3.9%,12.9%,7.0%,and 13.8%,compared with T1. In sum,the upper leaves development of flue-cured tobacco can be promoted significantly through warming properly after toping.

Key words: flue-cured tobacco, warming after toping, upper leaf, growth and development

HE Wen-jun, YANG Tie-zhao, TIAN Pei, DUAN Du-wei, LI Xiao-hui, HE Jia, ZHOU Jian-fei. Effect of Warming After Toping on the Growth and Development of the Upper Leaves of Flue-cured Tobacco[J]. Biotechnology Bulletin, 2019, 35(3): 19-23.

References

[1] 许自成, 黄平俊, 苏富强, 等. 不同采收方式对烤烟上部叶内在品质的影响[J]. 西北农林科技大学学报:自然科学版, 2005, 33(11):13-17.
[2] 宫长荣, 刘霞, 宋朝鹏, 等. 影响烤烟上部叶质量的因素及提高其可用性的措施[J]. 中国农学通报, 2007(3):103-108.
[3] 梁斌, 蔚应俊, 周应兵. 烤烟上部叶滞销的原因及农业生产对策[J]. 安徽农业科学, 2002, 30(2):285-286.
[4] 纪成灿, 王胜雷, 许锡祥. 提高上部叶可用性和降低上部叶比例的农业措施[J]. 中国烟草科学, 2001, 22(4):19-22.
[5] 唐经祥, 何厚民, 江理论. 关键农业技术措施对烤烟上部叶外观质量及经济性状的影响[A]//陈江华. 中国烟叶学术论文集[C]. 北京:科学技术出版社, 2004:107-109.
[6] 刘碧荣. 五项措施对烤烟上、中部叶烟碱及其它主要化学成分的影响[D]. 贵阳:贵州大学, 2006.
[7] 邱标仁, 林桂华. 提高龙岩烟区上部叶可用性的途径[J]. 中国烟草科学, 2000, 21(2):16-18.
[8] 周越, 范幸龙, 周冀衡, 等. 夜温升高对云南省高海拔烤烟理化特性及产、质量的影响[J]. 中国生态农业学报, 2015, 23(1):61-68.
[9] 王佩, 尤磊, 刘朝科, 等. 成熟期地温对烟叶主要致香物质含量的影响[J]. 安徽农业科学, 2014, 42(20):6569-6572.
[10] 聂荣邦, 韦建玉, 汪少波, 等. 烤烟上部叶遮荫处理对田间小气候及烟叶质量的影响[J]. 作物研究, 2013, 27(6):611-613.
[11] Maehly AC, Chance B.Catalases and peroxidases, part II[J]. Methods of Biochemical Analysis, 1954, 1(3):357-424.
[12] 刘晶晶, 邓泽元. 植物细胞壁中的伸展蛋白[J]. 氨基酸和生物资源, 2003(3):20-23.
[13] Cleland R.Hydroxyproline as an inhibitor of auxin-induced cell elongation[J]. Nature, 1963, 200(490):908.
[14] Roberts K, Shirsat AH.Increased extensin levels in Arabidopsis affect inflorescence stem thickening and height[J]. Journal of Experimental Botany, 2006, 57(3):537.
[15] Cleland R, Karlsnes AM.A possible role of hydroxyproline-containing proteins in the cessation of cell elongation[J]. Plant Physiology, 1967, 42(5):669-671.
[16] 郭联华, 尹佟明, 李淑娴, 等. 植物细胞壁伸展蛋白研究述评[J]. 林业科学, 2010, 46(12):144-152.
[17] 李雄彪, 杨中汉. 伸展蛋白的结构、功能、交联和生物合成[J]. 植物生理学通讯, 1990(3):7-13.
[18] 甘苏生, 沈曾佑, 张志良, 等. 黄瓜子叶细胞壁扩大生长机理的研究[J]. 植物学报, 1987(4):379-387.
[19] 黄海, 沈曾佑, 张志良, 等. 细胞壁中富含羟脯氨酸蛋白质与烟草叶细胞扩大生长的关系[J]. 植物生理学报, 1983(1):85-92.
[20] Cosgrove DJ.Loosening of plant cell walls by expansins[J]. Nature, 2000, 407(6802):321-326.
[21] 赵庆新, 袁生. 植物细胞壁研究进展[J]. 生物学通报, 2007(7):8-9.
[22] Lamport DTA.13-Structure and function of plant glycoproteins
[J]. Carbohydrates Structure & Function, 1980, 67(6):501-541.
[23] 王家利, 刘冬成, 郭小丽, 等. 生长素合成途径的研究进展[J]. 植物学报, 2012, 47(3):292-301.
[24] 原牡丹, 侯智霞, 翟明普, 等. IAA分解代谢相关酶(IAAO、POD)的研究进展[J]. 中国农学通报, 2008(8):88-92.