Biotechnology Bulletin ›› 2019, Vol. 35 ›› Issue (3): 6-12.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0788

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Integration Analysis of Exogenous Gene and Line-Specific Detection in the Insect-Resistant cry1C-Transgenic Rice Jishengjing3

JIN Yong-mei, MA Rui, YU Zhi-jing, LIN Xiu-feng   

  1. Agro-Biotechnology Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130124
  • Received:2018-09-10 Online:2019-03-26 Published:2019-04-03

Abstract: The flanking sequences of exogenous gene conferring insect-resistant cry1C-transgenic rice line Jishengjing3 and its inserted sites were analyzed by genome walking PCR,and a line-specific PCR detection method was established. T-DNA was found to be in nucleotides 2 790 685 to 2 790 589(GenBank accession number:NC_029257.1)in the chromosome 2 of Oryza Sativa(Japonica)genome after aligning the flanking sequences in left and right borders of Jishengjing3’s exogenous gene with the rice’s genome and T-DNA sequences. Based on the T-DNA insertion site,specific primers in the 2-sides region of the inserted site and the left border of T-DNA region were designed,and a event-specific PCR detection method for Jishengjing3 was established,which may provide accurate and rapid detection approach for identifying Jishengjing3.

Key words: insect-resistance cry1C-transgenic rice, flanking sequences, insertion site, event-specific detection