The Related Transcriptional Factors in the Transcriptome of Mechanically-damaged Zoysia japonica

doi:10.13560/j.cnki.biotech.bull.1985.2018-1052

Abstract

Abstract: This work aims to obtain differential genes（DEGs）related to AP2/EREBP,NAC,and WRKY transcription factors caused by mechanical damage. These three transcription factors were screened by converting the assembled sequences after transcriptome sequencing into Mapman-recognizable probe IDs,and were annotated from the Swiss-Prot and Nr databases in the transcriptome. Total 13 DEGs of AP2/EREBPs,8 DEGs of NACs and 11 DEGs of WRKYs were obtained. In contrast to control T7（Mechanically damaged 0 h）,one DEG was down-regulated in AP2/EREBPs,NACs,and WRKYs at T8（Mechanically damaged 2 h）and T9（Mechanically damaged 6 h）,respectively;whereas two DEGs of NAC showed up-regulated trend at T8 and down-regulated trend at T9,and the DEGs of other transcriptional factors were all up-regulated at T8 and T9. The database annotation indicated that AP2/EREBP and WRKY transcription factors enhanced the response to abiotic stress and biotic stress,i.e.,enhanced its stress resistance,when Zoysia japonica was damaged mechanically. Moreover,the NAC transcription factor transmitted signals to shoot apical meristem and regulated its growth.

Key words: Zoysia japonica, transcription factor, mechanical damage

ZHAO Fang-dong, ZENG Hui-ming. The Related Transcriptional Factors in the Transcriptome of Mechanically-damaged Zoysia japonica[J]. Biotechnology Bulletin, 2019, 35(4): 7-12.

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