Screening and Identification of High Uricase-producting Strain from Marine and the Enzymatic Properties

doi:10.13560/j.cnki.biotech.bull.1985.2018-1057

Abstract

Abstract: Uricase is used in routine clinical analysis and clinical medicine widely. Its importance in medical treatment and diagnosis is increasing. A high uricase-producting strain Z7 was screened from the Dalian Huanghai Sea mud and seawater by transparent circle method and enzyme coupling spectrophotometry,and identified as Bacillus fastidiosus by 16S rDNA phylogenetic analysis,morphology and physiological and biochemical characteristics. Results showed that the molecular weight of the protein was about 33 kD. The optimum temperature for uricase was 25℃,and specific activity was 673.7 U/mg . The optimum pH for uricase was 8.0,and it was stable in the pH range of 8.0 to 9.0. Its relative enzyme activity could still maintain more than 90% after 1 h’s incubation. The activation of Fe³⁺and Ca²⁺ on uricase was stimulative effects,while Ag⁺,Hg²⁺ showed strong inhibitory effects. Owing to the excellent chill enzyme activity and stability,Z7 can lay a theoretical foundation for the industrialized production of uricase and has potential development value.

Key words: Bacillus fastidious, identification, uricase, isolation and purification, enzymatic properties

ZHANG Qing-fang, PANG Fei, YU Shuang, XIAO Jing-hui, DOU Shao-hua, CHI Nai-yu. Screening and Identification of High Uricase-producting Strain from Marine and the Enzymatic Properties[J]. Biotechnology Bulletin, 2019, 35(7): 61-69.

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