Effects of Roundup Ready Soybean AG5601 on the Functional Diversity of Microbial Community in Rhizospheric Soil

doi:10.13560/j.cnki.biotech.bull.1985.2019-0002

Abstract

Abstract: This study aims to clarify the effects of Roundup Ready Soybean AG5601 on the functional diversity of microbial community in rhizospheric soil,for supplying basic information for the safety evaluation of transgenic soybean. BIOLOG method was applied to compare the carbon source utilization characteristics of rhizospheric microorganism among AG5601,their parent SNK500,and ordinary cultivated soybean Zhonghuang 13 at mature and stubble stage. In the mature stage,the carbon source utilization capacity of rhizospheric microbial community in AG5601 was significantly higher than that in SNK500;however,had no significant difference with that in Zhonghuang 13. At the stubble stage,there were no significant differences in the carbon source utilization capacity of rhizospheric microbial community among G5601,SNK500 and Zhonghuang 13. Functional diversity analysis found that the rhizosphere soil microbial diversity indices of Simpson（1/D）and Mcintosh（U）of AG5601 were significantly higher than those of SNK500 in the mature stage. In the stubble stage the differences in microbial diversity among 3 soybeans rhizosphere soil were insignificant. Principal component analysis indicated that there existed same in the carbon source utilization by the microbes in rhizosphere soils of SNK500 and AG5601 at the mature and stubble stage,suggesting the non-difference in the carbon source utilization pattern of the microbes during these processes. This study revealed that the effects of Roundup Ready soybean AG5601 on the diversity of microbial community in the rhizospheric soil were not significant.

Key words: Roundup Ready Soybean, rhizospheric microorganism, opticaldensity, microbialdiversity

ZHANG Zhuo, LIU Mao-yan, WANG Pei, HUANG Wen-kun, LIU Er-ming, PENG Huan, PENG De-liang. Effects of Roundup Ready Soybean AG5601 on the Functional Diversity of Microbial Community in Rhizospheric Soil[J]. Biotechnology Bulletin, 2019, 35(7): 17-24.

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