Advances in the Techniques of Studying Translatome

doi:10.13560/j.cnki.biotech.bull.1985.2019-0006

Abstract

Abstract: High-throughput sequencing-based RNA-Seq has been widely used to detect the expression and regulation mechanism of genes in tissues and cells. However,published literature have shown that mRNA level cannot reflect the real abundance of the related protein,while translatome that studies the being-translated mRNAs is more correlated to the actual abundance of proteins than the transcriptome. Therefore,studying translatome is more theoretical and practical meaning than studying transcriptome. In this paper,we reviewed the main techniques in studying translatome,from the most classical polysome profiling to the recently developed ribosome profiling,ribosomal affinity-purification,and RNC-RNA-Seq,and further compared the advantages and limitations of each technique. We also briefly discussed the prospect of studying translatome for providing a reference in studying translatome.

Key words: translatome, polysome profiling, ribosome affinity-purification, ribosomalprofiling, RNC-mRNA-Seq

WEI Kang-ning, CUI Jun-xia, WANG Meng-lei, WANG Li, LI Yong-fang. Advances in the Techniques of Studying Translatome[J]. Biotechnology Bulletin, 2019, 35(7): 222-229.

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