Effects of Phosphorus Concentrations on Growth and Metabolism of Seawater Spirulina platensis

doi:10.13560/j.cnki.biotech.bull.1985.2019-0181

Abstract

Abstract: Phosphorus plays an important role in the growth and metabolism of microalgae. In order to explore the feasibility of regulating the accumulation of major metabolites in seawater Spirulina by adjusting phosphorus concentration,we analyzed the physiological and biochemical effects of different phosphorus concentrations on seawater Spirulina platensis by using K₂HPO₃·3H₂O as phosphorus resource and setting 5 phosphorus concentrations 0.005-0.04 g/L based on seawater Zarrouk medium. The result showed that biomass production and protein content of seawater S. platensis were enhanced along with the increase of phosphorus concentration,and the maximum were 0.48±0.02 g/L and 59.23±0.61%DW,respectively,at the concentration of 0.04 g/L. The total polysaccharide content decreased significantly and then tended to be stable with the increasing of phosphorus concentration,and the maximum was 25.96±1.61%DW at phosphate concentration of 0.005 g/L. The content of C-phycocyanin,allophycocyanin and total phycobiliproteins increased first and then decreased along with the increasing of phosphorus concentration,and the maximum reached at the phosphate concentration of 0.03 g/L by 14.56±0.99%DW,4.21±0.19%DW and 18.77±0.39%DW respectively. The content of chlorophyll a and carotenoids increased along with the increasing of phosphate concentration,with the maximum value of 1.01±0.01%DW and 0.35±0.02%DW,respectively. The variation of phosphorus concentration generally demonstrated no significant effect on total lipids content and fatty acids composition（P>0.05）. The results showed that low phosphorus concentration was beneficial to the accumulation of polysaccharides;while high phosphorus concentration was beneficial to the synthesis of protein and phycocyanin,chlorophyll and carotenoid,thus the synthesis and accumulation of the main high-value products of seawater Spirulina could be effectively adjusted by changing phosphorus concentration.

Key words: seawater Spirulina platensis, growth, protein, polysaccharide, phycocyanin

CHEN Hao, YANG Bing-jie, LI Tao, WU Hua-lian, WU Hou-bo, XIANG Wen-zhou. Effects of Phosphorus Concentrations on Growth and Metabolism of Seawater Spirulina platensis[J]. Biotechnology Bulletin, 2019, 35(8): 103-110.

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