Abstract: To identify a strain（L-1）isolated from a sick Pelteobagrus fulvidraco and analyze its drug resistance,the morphology,physiological and biochemical analysis,16S rDNA gene and 23S rRNA gene amplification,and phylogenetic tree construction were carried out,and its drug resistance was determined by drug susceptibility test and drug-resistant genes test. The results showed that the strain L-1 was Gram-negative bacterium in short rob. The reactions to gelatin liquefaction,aesculin and arabinose and so on were negative,while that to arginine,tryptophan and glucose and so on were positive. The length of sequence fragment by 16S rDNA gene amplification was 1 461 bp. BLAST alignment showed that the similarity between the splicing sequence of L-1 and Plesiomonas shigellode in NCBI was 99%. In the phylogenetic tree of the strain L-1,the strain L-1 was the closest to P. shigellode. Only the target band with a size of 280 bp was amplified from the L-1 via 23S rRNA,while all other strains did not. Thus L-1 was comprehensively determined to be P. shigellode. Antimicrobial susceptibility test showed that L-1 was sensitive to cefoperazone,centamicin,etc.,and was moderate to tetracycline,and was resistant to benzocillin,ampicillin and other drugs. Drug-resistant gene Sul2,TEM,ant（3''）-Ⅰa and aac（6'）-Ⅰb in the strain L-1 were detected,while Sul1,Sul3,aph（3'）-Ⅱa and aac（3）-Ⅱa were not.

Key words: Plesiomonas shigellode, identification, drug resistance genes, antimicrobial susceptibility test