Correlation Analysis Between Microsatellite Polymorphism and Production Traits in Black Fur Sheep in Min County

doi:10.13560/j.cnki.biotech.bull.1985.2019-0621

Abstract

Abstract: The genetic diversity of 10 microsatellite loci in the black fur sheep population of Min county and its correlations with production traits were analyzed,which provides a theoretical basis for the protection,utilization and traits improvement of the germplasm resources. A total of 48 black fur sheep of Min county at 12,24 and 36 months old were selected,respectively. Their body weight,body height,body length,chest width,chest depth and chest circumference were measured. Meanwhile,10 pairs of microsatellite primers,PCR and gel electrophoresis technology were used to detect the genetic diversity of the black fur sheep in Min county and to analyze the correlation between various gene types and production traits. A total of 112 alleles were detected at 10 microsatellite loci. The average alleles was 11.2,the average effective alleles was 4.450 8,the average observed heterozygosity was 0.521 8,the average expected heterozygosity was 0.765 7,the average polymorphism information content was 0.728 9;10 loci had various degrees of correlation with body weight,body height,body length,chest width,chest depth and chest circumference. The selected 10 microsatellite loci were polymorphic and could be used to evaluate the genetic resources of black fur sheep in Min county. In addition,the selected loci were correlated with production traits at different degrees,which could be used to guide the trait improvement and breeding of black fur sheep in Min county.

Key words: black fur sheep in Min county, microsatellite, production traits, association analysis

ZHANG Rui, LANG Xia, WU Jian-ping, WANG Cai-lian, LIANG Ting-yu. Correlation Analysis Between Microsatellite Polymorphism and Production Traits in Black Fur Sheep in Min County[J]. Biotechnology Bulletin, 2019, 35(11): 55-63.

References

[1] 刘伯河, 王成强, 张广. 岷县黑裘皮羊生产方式调查[J]. 甘肃畜牧兽医, 2018, 48(4):38-39.
[2] 郎侠, 赵志艳, 张广, 等. 岷县黑裘皮羊遗传资源现状及发展建议[J]. 甘肃农业科技, 2019(1):67-71.
[3] Arranz JJ, Bayón Y, San PF.Genetic relationships among Spanish sheep using microsatellites[J]. Animal Genetic, 2015, 29(6):435-440.
[4] 王斌, 昝林森, 余横伟, 等. 秦巴山区黄牛群体的微卫星DNA遗传多样性[J]. 农业生物技术学报, 2016, 24(2):233-244.
[5] Suh S, Kim Y, Cho C, et al.Assessment of genetic diversity, relationships and structure among Korean native cattle breeds using microsatellite markers[J]. Asian-Austral J Anim, 2014, 27(11):1548-1553.
[6] Tefiel H, Ata N, Chahbar M, et al.Genetic characterization of four Algerian goat breeds assessed by microsatellite markers[J]. Small Ruminant Reserve, 2018, 160:65-71.
[7] Kumar S, Dixit S, Verma N, et al.Genetic diversity analysis of the gohilwari breed of Indian goat(Capra hircus)using microsatellite markers[J]. American Journal of Animal and Veterinary Sciences, 2009, 4(3):49-57.
[8] 王可, 刘昭华, 崔绪奎, 等. 济宁青山羊微卫星标记多态性分析[J]. 山东农业科学, 2017, 49(2):142-146.
[9] 邱小宇, 黄勇富, 赵永聚, 等. 中国6个地方绵羊品种的微卫星分析[J]. 中国畜牧兽医, 2016, 43(4):1024-1031.
[10] Fang M, Hu X, Jiang T, et al.The phylogeny of Chinese indigenous pig breeds inferred from microsatellite markers[J]. Anim Genet, 2015, 36(1):7-13.
[11] 赵思思, 贾青, 胡慧艳, 等. 河北省4个黑猪群体的遗传多样性分析[J]. 河北农业大学学报, 2017, 40(2):79-83.
[12] 黄勋和, 赵雪敏, 柯振华, 等. 中国乌骨鸡微卫星位点LEI0258多态性研究[J]. 江苏农业科学, 2019, 47(5):40-45.
[13] 李红伟, 陈圆, 邓丹青, 等. 惠阳胡须鸡微卫星多态性研究[J]. 广东农业科学, 2017, 44(2):149-153.
[14] 王斌, 昝林森, 杜宝民, 等. 西镇牛微卫星DNA与生长发育性状的关联性分析[J]. 西北农林科技大学学报:自然科学版, 2017, 45(3):7-16.
[15] 王斌, 昝林森, 杜宝民, 等. 宣汉牛微卫星DNA与生长发育性状的关联分析[J]. 农业生物技术学报, 2018, 26(7):1174-1185.
[16] 决肯·阿尼瓦什, 韩业东, 李齐发, 等. 巴什拜羊微卫星标记多态性及其与生长指标关联性分析[J]. 中国农业科学, 2010, 43(16):3425-3432.
[17] 任坤刚, 于成江, 康康, 等. 4个微卫星座位多态性与萨福克羊体尺指标相关性的初步研究[J]. 西北农林科技大学学报:自然科学版, 2008, 36(1):43-48, 53.
[18] 梁庆玲, 牛志刚, 杨丹, 等. 陶赛特羊Carwell基因微卫星标记多态性与体重和体尺指标的相关性分析[J]. 中国草食动物, 2012, 32(1):12-16.
[19] 包文斌, 束婧婷, 许盛海, 等. 样本量和性比对微卫星分析中群体遗传多样性指标的影响[J]. 中国畜牧杂志, 2007, (1):6-9.
[20] 牛东红, 陈慧, 林国文, 等. 缢蛏群体微卫星分析中样本量对遗传多样性指标的影响[J]. 海洋科学进展, 2010, 28(2):203-208.
[21] Purvis WI, Franklin IR.Major genes and QTL influencing wool production and quality:A review[J]. Genet Sel Evol, 2005, 37(Suppl 1):S97-S107.
[22] 李碧春. 动物遗传学[M]. 北京:中国农业大学出版社, 2008.
[23] 王书珍, 黄兴学, 王斌才, 等. 生菜SSR遗传多样性及其与营养品质性状的关联分析[J]. 江苏农业科学, 2018, 46(24):161-164.
[24] 王玉琴, 张娜娜, 刘小芳, 等. 微卫星标记与太行黑山羊生长性状的相关研究[J]. 畜牧与兽医, 2011, 43(6):17-21.
[25] 刘德稳, 陈冰, 傅彤, 等. 鲁山牛腿山羊体尺性状与微卫星标记的相关分析[J]. 西南大学学报:自然科学版, 2011, 33(12):18-24.