The Genetic Diversity of Six Chinese Sheep Breeds by Six Microsatellite Markers

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.012

Abstract

Abstract: Six microsatellite loci were used to analyze the genetic diversity of 280 sheep from 6 Chinese breeds of Ujumqin sheep，Small-tailed Han sheep，Tan sheep，Zhaotong sheep，Hulun Buir sheep，and Gansu Alpine Merino. We calculated the polymorphism information content，heterozygosity and genetic distance，analyzed the principal components and clustered UPGMA from these 6 sheep breeds. Altogether，101 alleles were found，the mean heterozygosity was 0.599-0.691，and mean polymorphism information content was 0.609-0.680. The Gansu Alpine Merino sheep had the most genetic divergence compared to other sheep breeds，while the Hulun Buir and Ujumqin ones were the least in genetic divergence. The above results showed that these 6 breeds had relatively high genetic diversity，and their genetic relationships were consistent with their formation history，differentiation and geographical distribution.

Key words: microsatellite, genetic diversity, sheep

CHEN Li-peng,HUANG Yong-fu,ZHAO Yong-ju,E Guang-xin,NA Ri-su. The Genetic Diversity of Six Chinese Sheep Breeds by Six Microsatellite Markers[J]. Biotechnology Bulletin, 2016, 32(5): 91-98.

References

[1]马月辉, 吕慎金, 侯冠或, 等. 中国部分绵羊品种遗传多样性研究[C]//中国畜牧兽医学会养羊学分会全国养羊生产与学术研究会议论文集. 中国草食动物杂志出版社, 2005.
[2]岳远瑞, 袁晓航, 周路, 等. 新疆7个绵羊品种MHC区段微卫星遗传多样性的分析[J]. 石河子大学学报：自然科学版, 2014, 32（4）：438-443.
[3]Botstein D, White RL, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am J Hum Genet, 1980, 32：314-331.
[4] Lumsden JM, Loard EA, Montgomery GW. Characterisation and linkage mapping of ten microsatellite markers derived from a sheep x hamster cell hybrid[J]. Animal Genetics, 1996, 27：203-206.
[5] Chen HJ, Yue YS, Fan XZ, et al, Study comparatively on genetic distance and genus analysis measures in Shandong indigenous chicken breeds[J]. Journal of Farming and Veterinarian, 2004, 35（1）：33-36.
[6] Sun W, Chang H, Tsunoda K, et al. Analysis of geographic and pairwise genetic distances among sheep populations[J]. Biochem Genet, 2010, 48（5-6）：376-84.
[7]Agaviezor BO, Peters SO, Adefenwa MA, et al, Morphological and microsatellite DNA diversity of Nigerian indigenous sheep[J]. Anim Sci Biotechnol, 2012, （1）：38.
[8]Yilmaz O, Cemal I, Karaca O. Genetic diversity in nine native Turkish sheep breeds based on microsatellite analysis[J]. Anim Genet, 2014, 45（4）：604-608.
[9]Leroy G, Danchin-Burge C, Palhière I, et al. How do introgression events shape the partitioning of diversity among breeds：a case study in sheep[J]. Genet Sel Evol, 2015, 47（1）：48.
[10]?urkovi? M, Ramljak J, Ivankovi? S, et al. The genetic diversity and structure of 18 sheep breeds exposed to isolation and selection[J]. J Anim Breed Genet, 2016, 133（1）：71-80.
[11]曾盛诚. 利用20个微卫星标记分析国内外11个鸡群体的遗传多样性[D]. 北京：中国农业科学院, 2009.
[12]Park SDE. Trypanotolerance in West African Cattle and the population genetic effects of selection[D]. Dublin：University of Dublin, 2001.
[13]Excoffier L, Lischer HEL. Arlequin 3.5：A new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources, 2010, 10：564-567.
[14]Raymond M, Rousset F. GENEPOP（Version 1. 2）：population genetics software for exact tests and ecumenicism[J]. Journal of Heredity, 1995, 86（3）：248-249.
[15]Goudet J. STAT（Version 1. 2）：A computer program to Calculate F-Statistic[J]. J Hered, 1995, 86：485-486.
[16]Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proc Natl Acad Sci USA, 1979, 76：52-69.
[17] Nei M, Li WH. Analysis of gene diversity in subdivided populations[J]. Proc Natl Acad Sci USA, 1973, 70：3321-3323.
[18]常爽. 9个绵羊群体遗传多样性的微卫星分析[D]. 郑州：河南农业大学, 2010.
[19] Botstein D, White RL, Skolnick MH, Davis R. Construction of a genetic linkage map in man using restriction fragment length poly-morphisms[J]. Am J Hum Genet, 1980, 32：314-331.
[20] Ma YH, Rao SQ, Lu SJ, et al. Phylogeography and origin of sheep breeds in Northern China[J]. Conservation Genetics, 2006, 7（1）：117-127.
[21]储明星, 程金华, 过纬. 微卫星标记OarAE101和BM1329在五个绵羊品种中的初步研究[J]. 遗传学报, 2001, 28（6）：510- 517.
[22]吕慎金, 杨燕, 候冠玉, 等. 运用微卫星标记对中国地方绵羊品种的遗传多样性分析[J]. 畜牧兽医学报, 2008, 7：858-865.
[23]储明星, 王吉振, 王爱国, 等. 小尾寒羊五个微卫星基因座遗传多态性研究[J]. 遗传学报, 2002, 29（6）：502-506.
[24]李延璐. 7个绵羊群体15个微卫星DNA座位遗传多样性分析[D]. 合肥：安徽农业大学, 2009.
[25]赵有璋. 中国养羊学[M]. 北京：中国农业出版社, 2011.
[26]杜立新. 中国畜禽遗传资源志. 羊志[M]. 北京：中国农业出版社, 2011.
[27]仲涛, 马月辉, 关伟军, 等. 10 个绵羊品种的微卫星 DNA 多态性研究[J]. 畜牧兽医学, 2008, 39（5）：555-561.
[28]郭小雅, 杨章平, 常洪, 等. 13我国6个绵（山）羊群体遗传分化的微卫星分析[J]. 畜牧与兽医, 2007, 39（5）：16-20.
[29]任春环, 张子军, 吴建平, 等. 5个绵羊群体微卫星标记的遗传多态性分析[J]. 安徽农业大学学报, 2010, 37（3）：436-439 .
[30]兰蓉, 洪琼花, 张俊, 等. 云南不同地区绵羊血液蛋白多态性研究[J]. 草食家畜, 2002, 3（1）：43-47.
[31]仲涛. 中国部分绵羊品种的遗传多样性研究[D]. 北京：中国农业科学院, 2007.
[32]娄渊根, 刘丑生, 刘建学, 等. 9个绵羊品种微卫星DNA遗传多样性分析[J]. 中国畜牧兽医, 2011, 38（10）：106-112.