Expression of Recombinant Ovine FSH in the CHO-K1 Cell and Its Application

doi:10.13560/j.cnki.biotech.bull.1985.2019-0668

Abstract

Abstract: This work aims to explore the expression of recombinant sheep FSH（follicle stimulating hormone）in CHO-K1 cells and its biological activity. The recombinant plasmid pcDNA3.1-FSHβα was constructed and electroporated into fully suspended CHO-K1 cells. The monoclonal cells with high expression of recombinant FSH protein were screened under G418 pressure,cultured in a bioreactor and concentrated by ultrafiltration. The concentrated recombinant sheep FSH was injected into mice,the ovarian mass of mice was weighed,and the contents of luteinizing hormone and progesterone in mouse serum were detected. The recombinant plasmid pcDNA3.1-FSHβα was successfully constructed and the secretory expression was successful in CHO-K1 cells,and the expression level was 297.97 ng/mL. The ovaries of mice injected with concentrated recombinant sheep FSH protein were significantly enlarged,and the contents of luteinizing hormone and progesterone were also significantly up-regulated. In sum,the recombinant sheep FSH protein is successfully expressed in CHO-K1 cells and presents certain biological activity.

Key words: follicular stimulating hormone, CHO-K1 cells, bioreactor

WANG Xing-long, LI Qian, MA Rui-xian, LI Sheng-jun, LI Xiang-rong, FENG Ruo-fei. Expression of Recombinant Ovine FSH in the CHO-K1 Cell and Its Application[J]. Biotechnology Bulletin, 2019, 35(12): 112-117.

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