Analysis of Fresh-cut Shiitake（Lentinula edodes）and Autolysis During Storage

doi:10.13560/j.cnki.biotech.bull.1985.2019-0781

Abstract

Abstract: This study is aimed to analyze irritable physiological change and cellular structure variation of Lentinula edodes after fresh-cut process. The fruiting body of shiitake 0912 was chosen as object in this study. The changing trend of physiological indexes at low temperature storage and room temperature storage conditions were investigated. The variation of cellular structure in aging tissue was detected by microtechnic including optical microscopy,fluorescence microscopy and transmission electron microscopy. The results showed that low temperature storage was beneficial to reduce the rate of browning,softening and weight losing,also to slow down the decline of antioxidant capacity. Microstructure analysis indicated that autolysis phenomenon occurred in the tissues after mechanical damage,such as cell wall breakage,membrane ablation,self-phagocytosis,DNA fragmentation,nucleus disappearance,and cell apoptosis. The autolysis phenomenon in fresh-cut shiitake occurs,and the damage of cellular structures is one of the important factors that induce the deterioration of shiitake,and accelerates the rotten progress with storage environment.

Key words: fresh-cut, Lentinula edodes, autolysis, microanalysis

ZHAO Shuang, SU Zhe, GU Tong-tong, GAO Qi, RONG Cheng-bo, SONG Shuang, LIU Yu. Analysis of Fresh-cut Shiitake（Lentinula edodes）and Autolysis During Storage[J]. Biotechnology Bulletin, 2020, 36(5): 176-182.

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