Effects of Different Cultivation Substrates on the Laccase Activities of Lentinula edodes During Liquid Fermentation

doi:10.13560/j.cnki.biotech.bull.1985.2021-0844

Abstract

Abstract:

The aims are to explore the laccase-producing capacities of different Lentinula edodes strains in Guizhou and obtain a high-yield laccase cultivation substrate,and therefore lay a theoretical foundation for increasing and improving the yield and quality of L. edodes,for achieving the purpose of revitalizing the mushroom industry in Guizhou. Taking Guizhou L. edodes 808,Qingke R20 and Guizhou’s unique Masang L. edodes as the research objects,the discriminative identification of the three species of L. edodes strains was preliminarily studied through antagonistic reaction,phylogenetic tree and temperature tests. And the main cultivation substrates of L. edodes were used as an inducer,the changing pattern on the laccase production activities of three different L. edodes strains in liquid fermentation for 12 consecutive days were studied. Although Masang L. edodes,L. edodes 808 and Qingke R20 were all subordinate branches of organic L. edodes,and there were differences among the strains,meaning there were certain provenance differences. The liquid laccase activity of L. edodes was significantly affected by the genetic differences of the strains and the 4 different induction media（P<0.001）,and the laccase activity showed a trend of increasing first and then decreasing under each treatment. The laccase activity of L. edodes was lower under all treatments in the early stage of culture,higher in the middle and late stages of culture,and the peak value was mainly concentrated in the 8-11 d. Compared with the strain Qingke R20 and Masang L. edodes,L. edodes 808 had the strongest liquid laccase-producing capacity in the induction medium containing corn cob,Coriariane palensis sawdust and Quercus acutissima sawdust,and their maximum enzyme activity was 464.06,238.64 and 288.94 U/L,respectively. However,wheat bran was more inducible to Qingke R20,with the maximum enzyme activity reaching 265.07 U/L. In addition,wheat bran presented the most concentrated and rapid response time for inducing laccase production from Masang L. edodes,with the maximum enzyme activity reaching 161.83 U/L. However,the high level of inducibility was more durable in C. palensis sawdust,with the maximum enzyme activity reaching 108.61 U/L. In conclusion,there are differences among the three strains of Masang L. edodes,L. edodes 808 and Qingke R20,meaning that there were certain provenance differences. From the perspective of laccase production level,L. edodes 808,Qingke R20 and Masang L. edodes had great differences in lignin degradation and absorption capacity of 4 substrates,and their best substrates were corn cob,wheat bran and C. palensis sawdust,respectively.

Key words: Lentinula edodes, distinctiveness, cultivation substrate, liquid fermentation, laccase activity

XIONG Xue, LI Peng, ZHANG Gui-he, XIANG Zhun, TAO Wen-Guang, ZHOU Guang-yan, HE Yao-wei. Effects of Different Cultivation Substrates on the Laccase Activities of Lentinula edodes During Liquid Fermentation[J]. Biotechnology Bulletin, 2021, 37(12): 50-59.

Figures/Tables 9

References 24

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菌株Strain	马桑香菇 Masang L. edodes	香菇808 L. edodes 808	庆科R20 Qingke R20
马桑香菇 Masang L. edodes	-
香菇808 L. edodes 808	+	-
庆科R20 Qingke R20	+	-	-

菌株Strain	马桑香菇 Masang L. edodes	香菇808 L. edodes 808	庆科R20 Qingke R20
马桑香菇 Masang L. edodes	-
香菇808 L. edodes 808	+	-
庆科R20 Qingke R20	+	-	-

菌株 Strain	温度 Temperature/℃	萌发时间Germination time/h	菌落长势Colony growth	菌落描述 Colony description	日均生长速度 Average daily growth rate/（cm·d^-1）	差异显著性 Significant difference （P<0.05）
马桑香菇 Masang L. edodes	10	48	++	绒毛状白色圆形菌落,边缘整齐	0.11	e
	15	48	+++	绒毛状白色圆形菌落,边缘整齐	0.34	d
	20	48	++++	绒毛状白色圆形菌落,边缘整齐	0.49	b
	25	48	++++	绒毛状白色圆形菌落,边缘整齐	0.89	a
	30	48	+++	绒毛状白色圆形菌落,边缘整齐	0.41	c
香菇808 L. edodes 808	10	48	+	绒毛状白色圆形菌落,边缘整齐	0.15	d
	15	48	++	绒毛状白色圆形菌落,边缘整齐	0.28	c
	20	48	+++	绒毛状白色圆形菌落,边缘整齐	0.44	b
	25	48	++++	绒毛状白色圆形菌落,边缘整齐	0.85	a
	30	48	++++	绒毛状白色圆形菌落,边缘整齐	0.31	c
庆科R20 Qingke R20	10	48	++	绒毛状白色圆形菌落,边缘整齐	0.10	e
	15	48	+++	绒毛状白色圆形菌落,边缘整齐	0.29	c
	20	48	+++	绒毛状白色圆形菌落,边缘整齐	0.50	b
	25	48	++++	绒毛状白色圆形菌落,边缘整齐	0.89	a
	30	48	++	绒毛状白色圆形菌落,边缘较整齐	0.25	d

菌株 Strain	温度 Temperature/℃	萌发时间Germination time/h	菌落长势Colony growth	菌落描述 Colony description	日均生长速度 Average daily growth rate/（cm·d^-1）	差异显著性 Significant difference （P<0.05）
马桑香菇 Masang L. edodes	10	48	++	绒毛状白色圆形菌落,边缘整齐	0.11	e
	15	48	+++	绒毛状白色圆形菌落,边缘整齐	0.34	d
	20	48	++++	绒毛状白色圆形菌落,边缘整齐	0.49	b
	25	48	++++	绒毛状白色圆形菌落,边缘整齐	0.89	a
	30	48	+++	绒毛状白色圆形菌落,边缘整齐	0.41	c
香菇808 L. edodes 808	10	48	+	绒毛状白色圆形菌落,边缘整齐	0.15	d
	15	48	++	绒毛状白色圆形菌落,边缘整齐	0.28	c
	20	48	+++	绒毛状白色圆形菌落,边缘整齐	0.44	b
	25	48	++++	绒毛状白色圆形菌落,边缘整齐	0.85	a
	30	48	++++	绒毛状白色圆形菌落,边缘整齐	0.31	c
庆科R20 Qingke R20	10	48	++	绒毛状白色圆形菌落,边缘整齐	0.10	e
	15	48	+++	绒毛状白色圆形菌落,边缘整齐	0.29	c
	20	48	+++	绒毛状白色圆形菌落,边缘整齐	0.50	b
	25	48	++++	绒毛状白色圆形菌落,边缘整齐	0.89	a
	30	48	++	绒毛状白色圆形菌落,边缘较整齐	0.25	d

培养时间 Incubation period/d	漆酶活性Laccase activity/（U·L^-1）
培养时间 Incubation period/d	菌株 Strain	培养基 Substrate	菌株×培养基 Strain × Substrate
2	65.718^***	23.380^***	6.887^***
3	38.515^***	26.703^***	5.890^***
4	23.114^***	30.396^***	21.908^***
5	40.804^***	31.882^***	31.989^***
6	59.004^***	24.854^***	41.526^***
7	212.19^***	79.513^***	80.377^***
8	353.451^***	133.537^***	111.936^***
9	302.412^***	96.631^***	67.755^***
10	537.164^***	143.962^***	115.817^***
11	371.454^***	164.037^***	91.993^***
12	93.704^***	45.079^***	34.453^***