Effect of Morchella Mycelium on Soil Enzymatic Activity

doi:10.13560/j.cnki.biotech.bull.1985.2020-0090

Abstract

Abstract: This work aims to study on the effect of Morchella mycelium on soil enzymatic activity. The sucrase and amylase activities of soil samples were determined by 3,5-dinitrosalicylic acid colorimetric method with single factor and orthogonal experimental design. The optimal condition of the soil sucrase activity was that the temperature was 28℃,the time was 3 d,the amount of added mycelium was 1∶5,and the soil sucrase activity increased 12 times compared with the control group. The optimal condition of soil amylase activity was as such：the amount of added mycelium was 1∶5,the temperature was 28℃,the time was 7 d,and the soil amylase activity increased 18 times compared with the control group. Morchella mycelium can improve the sucrase and amylase activities in the soil in a short period of time,which is of significance for improving soil.

Key words: Morchella mycelium, sucrase, amylase

WEI Jing-jing, ZHANG Hao-ran, WANG Zhi-ge, WANG Hui-chun. Effect of Morchella Mycelium on Soil Enzymatic Activity[J]. Biotechnology Bulletin, 2020, 36(9): 211-217.

References

[1] 杜习慧, 赵琪, 杨祝良. 羊肚菌的多样性、演化历史及栽培研究进展[J]. 菌物学报, 2014, 33(2):183-197.
Du XH, Zhao Q, Yang ZL.The diversity, evolution history and cultivation research progress of morel[J]. Acta Mycology, 2014, 33(2):183-197.
[2] 李娟, 王臻, 姚良同, 等. 羊肚菌多糖研究进展[J]. 微生物学杂志, 2005(4):89-91.
Li J, Wang Z, Yao LT, et al.Research progress of morel polysaccharides[J]. Journal of Microbiology, 2005(4):89-91.
[3] 赵苗. 羊肚菌生长过程中土壤酶变化规律研究[C]. 中国菌物学会、迪庆藏族自治州人民政府. 2018第三届全国羊肚菌大会资料汇编, 2018:113-120.
Zhao M.Study on the changes of soil enzymes during the growth of morel[C]. Chinese Society of Mycology, People’s Government of Diqing Tibetan Autonomous Prefecture. 2018 third National Morel Conference. Chinese Society of Mycology, 2018:113-120.
[4] 申时才, 朱映安, 钱洁, 等. 香格里拉县五境乡传统人工种植羊肚菌对经济生态的影响[J]. 西南农业学报, 2011, 24(5):2004-2009.
Shen SC, Zhu YA, Qian J, et al.The impact of traditional artificial cultivation of morels on economic ecology in Wujing township, Shangri-La county[J]. Journal of Southwest Agriculture, 2011, 24(5):2004-2009.
[5] 张猛. 土壤管理方式对李园土壤影响研究[D]. 成都:四川农业大学, 2004.
Zhang M.Study on the influence of soil management methods on the soil of Liyuan garden[D]. Chengdu:Sichuan Agricultural University, 2004.
[6] 赵苗, 张能, 谢敬宜, 等. 羊肚菌生长过程中土壤酶变化规律研究[J]. 中国食用菌, 2017, 36(2):41-46.
Zhao M, Zhang N, Xie JY, et al.Study on the change law of soil enzymes during the growth of morchella[J]. Chinese Edible Fungus, 2017, 36(2):41-46.
[7] 刘纪爱, 束爱萍, 刘光荣, 等. 施肥影响土壤性状和微生物组的研究进展[J]. 生物技术通报, 2019, 35(9):21-28.
Liu JA, Shu AP, Liu GR, et al.Research progress in the effects of fertilization on soil properties and microbiome[J]. Biotechnology Bulletin, 2019, 35(9):21-28.
[8] 万忠梅, 宋长春. 土壤酶活性对生态环境的响应研究进展[J]. 土壤通报, 2009, 40(4):951-956.
Wan ZM, Song CC.Research progress on the response of soil enzyme activity to ecological environment[J]. Soil Bulletin, 2009, 40(4):951-956.
[9] 邵文山, 李国旗. 土壤酶功能及测定方法研究进展[J]. 北方园艺, 2016(9):188-193.
Shao WS, Li GQ.Soil enzyme function and determination method research progress[J]. Northern Horticulture, 2016(9):188-193.
[10] 周玮, 周运超, 李进. 花江石漠化喀斯特土壤有机碳及其部分转化酶演变[J]. 农业现代化研究, 2007(1):110-113.
Zhou W, Zhou YC, Li J.The evolution of organic carbon and some invertases in Huajiang rocky desertification karst soil[J]. Research of Agricultural Modernization, 2007(1):110-113.
[11] 季向阳, 罗孝坤. 羊肚菌液体培养条件的优化[J]. 中国食用菌, 2016, 35(2):21-23, 27.
Ji XY, Luo XK.Optimization of liquid culture conditions of morel mushroom[J]. Chinese Edible Fungus, 2016(2):21-23, 27.
[12] 王莹, 孙永海, 王笑丹, 等. 粗柄羊肚菌菌丝体液体培养条件的优化[J]. 食用菌, 2007(6):6-8.
Wang Y, Sun YH, Wang XD, et al.Optimization of liquid culture conditions for the mycelium of morchella crassicarpa[J]. Edible Fungus, 2007(6):6-8.
[13] 敬华英. 羊肚菌种属鉴定及活性成分保健功效研究进展[J]. 安徽农业科学, 2018, 46(14):34-36, 140.
Jing HY.Research progress on the identification of morel and the health benefits of its active ingredients[J]. Anhui Agricultural Sciences, 2018, 46(14):34-36, 140.
[14] 郭晓蕾. 羊肚菌发酵液及其胞外多糖的分析与研究[D]. 长春:吉林大学, 2016.
Guo XL.Analysis and research of morel fermentation broth and its extracellular polysaccharide[D]. Changchun:Jilin University, 2016.
[15] 俞明义, 龚建锋. DNS法对金耳注射液多糖含量的测定[J]. 浙江中医杂志, 2006(4):235.
Yu MY, Gong JF.Determination of Polysaccharides in Jiner Injection by DNS method[J]. ZheJiang Journal of Traditional Chinese Medicine, 2006(4):235.
[16] 赵凯, 许鹏举, 谷广烨. 3, 5-二硝基水杨酸比色法测定还原糖含量的研究[J]. 食品科学, 2008(8):534-536.
Zhao K, Xu PG, Gu GY.Study on 3, 5-Dinitrosalicylic acid colorimetric determination of reducing sugar content[J]. Food Science, 2008(8):534-536.
[17] 韩玮, 孙晨曦, 苏敬. 模拟增温和酸雨对水稻土酶活性及温度敏感性的影响[J]. 生态与农村环境学报, 2017, 33(12):1117-1124.
Han W, Sun CX, Su J.Effects of simulated warming and acid rain on enzyme activities and temperature sensitivity of paddy soil[J]. Journal of Ecology and Rural Environment, 2017, 33(12):1117-1124.
[18] Yang QP, Xu M, Liu HS, et al.Impact factors and uncertainties of the temperature sensitivity of soil respiration[J]. Acta Ecologica Sinica, 2011, 31(8):2301-2311.
[19] 李娜, 王根绪, 高永恒, 等. 模拟增温对长江源区高寒草甸土壤养分状况和生物学特性的影响研究[J]. 土壤学报, 2010, 47(6):1214-1224.
Li N, Wang GX, Gao YH, et al.Effects of simulated temperature increase on soil nutrient status and biological characteristics of alpine meadows in the source area of the Yangtze River[J]. Acta Pedologica Sinica, 2010, 47(6):1214-1224.
[20] 李天星. 羊肚菌大田无基料栽培技术[J]. 农村经济与科技, 2019, 30(13):82-83.
Li TX.Cultivation techniques of morels without foundation in the field[J]. Rural Economy and Technology, 2019, 30(13):82-83.
[21] 王艮梅, 黄松杉, 郑光耀, 等. 菌渣作为土壤调理剂资源化利用的研究进展[J]. 土壤通报, 2016, 47(5):1273-1280.
Wang GM, Huang SS, Zheng GY, et al.Research progress on the utilization of bacterial residues as soil conditioners[J]. Soil Bulletin, 2016, 47(5):1273-1280.
[22] 王莹, 马宏伟. 食用菌废渣改良土壤理化性质的研究[J]. 吉林农业, 2013(1):59-60.
Wang Y, Ma HW.Study on the improvement of soil physical and chemical properties by edible fungus residue[J]. Jilin Agriculture, 2013(1):59-60.
[23] 唐凯悦, 尹蕾, 张跃新, 等. 木耳栽培废弃料改良土壤应用研究[J]. 牡丹江师范学院学报:自然科学版, 2016(1):68-69.
Tang KY, Yin L, Zhang YX, et al.Study on the application of fungus cultivation waste materials to improve soil[J]. Journal of Mudanjiang Teachers College:Natural Science Edition, 2016(1):68-69.
[24] 滕青, 曾梦凤, 林慧凡, 等. 菌渣还田对生菜生长、土壤养分及酶活性的影响研究[J]. 中国农学通报, 2020, 36(6):30-36.
Teng Q, Zeng MF, Lin HF, et al.Effects of returning bacterial residues to the field on lettuce growth, soil nutrients and enzyme activities[J]. Chinese Agricultural Science Bulletin, 2020, 36(6):30-36.