Expression and Analysis of an Amylase with Agarase Activity from a Marine Bacterium

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.018

Abstract

Abstract: Our aim is to confirm that there is amylase with agarase activity in the novel marine bacterial strain ZC1. The amylase gene amy440 of strain ZC1 was cloned and expressed in recombinant prokaryotic vector and the amylase and agarase activity of the recombinant protein was determined using the method of activity staining electrophoresis. Results indicated that the recombinant protein of gene amy440 showed amylase and agarase activity in the activity staining electrophoresis. Conclusively,the encoded protein of gene amy440 of strain ZC1 is an amylase with agarase activity.

Key words: agarase, amylase, multifunctional enzyme, gene cloning, recombinant expression

LIN Bo-kun, SONG Yan, LU Guo-yong, ZHAO Min, ZHONG Ming-qi, HU Zhong. Expression and Analysis of an Amylase with Agarase Activity from a Marine Bacterium[J]. Biotechnology Bulletin, 2017, 33(2): 125-130.

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