Abstract:

The objective of this study is to screen phosphorus-dissolving bacteria with fine environment adaptability to coastal salt-alkali land. Strain Y2R2 with the ability of salt-alkali-tolerant and phosphorus-dissolving was isolated from salt-alkali soil samples collected from Tianjin Binhai New Area. Strain Y2R2 was identified as a novel species of Halomonas according to poly-phase taxonomic characteristics. Strain Y2R2 grew at the temperature 10℃ to 45℃,and tolerated the condition of pH 14 and 15% NaCl. Strain Y2R2 showed ability of dissolving phosphorus under pH 7-13 and NaCl content 0-12%. In inorganic phosphorus medium,under the fermentation conditions in which the initial pH was 8,NaCl content was 9% and fermentation time was 48 h,the phosphate-dissolving quantity by strain Y2R2 reached its highest value,247.6 mg/L. Strain Y2R2 screened in this study is a novel species of indigenous microorganisms that adapted to the soil and climate environment of coastal salt-alkali land,and has high salt-alkali tolerance and phosphate-dissolving capacities. The strain is expected to be used as salt-alkali phosphorus-dissolving bacterial agent.

Key words: salt-alkali-tolerant phosphorus dissolving bacteria, taxonomic identification, Halomonas, novel species