Screening and Identification of High-yield Feruloyl Esterase Strains and Optimizing of the Enzyme Activity Assay Conditions

doi:10.13560/j.cnki.biotech.bull.1985.2020-0304

Abstract

Abstract:

Using transparent zone and solid fermentation,two strains with high feruloyl esterase（FAE）yield were selected from 106 macrofungi that were collected from field and deposited in the lab an. They were identified as Cerrena unicolor and Pycnoporus sanguineus by ITS sequence analysis and morphological identification. Then the FAE activity of two screened strains were determined using rice bran as induction medium and the crude enzyme with the highest activity was selected separately to optimize the enzyme activity determination conditions. The results demonstrated that the activity of FAE by C. unicolor and P. sanguineus reached the peak at 4 d and 6 d respectively. The optimal determination conditions for FAE activity in the crude enzyme solution of C. unicolor was 40℃,25 min reaction time,30 μL crude enzyme and 400 μL the methyl ferulate. The optimal determination conditions for FAE activity in the crude enzyme solution of P. sanguineus was 30℃,35 min reaction time,20 μL crude enzyme solution and 400 μL methyl ferulate.

Key words: macrofungi, feruloyl esterase, screening and identification, determination of enzyme activity

WANG Xiang-feng, WANG Qiao, YUAN Hui-jun, WANG Li. Screening and Identification of High-yield Feruloyl Esterase Strains and Optimizing of the Enzyme Activity Assay Conditions[J]. Biotechnology Bulletin, 2020, 36(10): 135-141.

Figures/Tables 9

References 26

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